Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields   Including Evidences from Cell and Animal Toxicity

doi:10.1038/srep18781

CORC > 北京大学 > 环境科学与工程学院

	Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity
	Wei, Kai ; Li, Wei ; Gao, Shan ; Ji, Bin ; Zang, Yating ; Su, Bo ; Wang, Kaile ; Yao, Maosheng ; Zhang, Jue ; Wang, Jinglin
刊名	SCIENTIFIC REPORTS
	2016
关键词	AEROSOLIZED RICIN INDUCE APOPTOSIS RHESUS MACAQUES BALB/C MICE INHALATION PROTEINS THERAPY CHAIN INHIBITION PROTECTION
DOI	10.1038/srep18781
英文摘要	Ricin is one of the most toxic and easily produced plant protein toxin extracted from the castor oil plant, and it has been classified as a chemical warfare agent. Here, nanosecond pulsed electric fields (nsPEFs) at 30 kV/cm (pulse durations: 10 ns, 100 ns, and 300 ns) were applied to inactivating ricin up to 4.2 mu g/ mL. To investigate the efficacy, cells and mice were tested against the ricin treated by the nsPEFs via direct intraperitoneal injection and inhalation exposure. Results showed that nsPEFs treatments can effectively reduce the toxicity of the ricin. Without the nsPEFs treatment, 100% of mice were killed upon the 4 mu g ricin injection on the first day, however 40% of the mice survived the ricin treated by the nsPEFs. Compared to injection, inhalation exposure even with higher ricin dose required longer time to observe mice fatality. Pathological observations revealed damages to heart, lung, kidney, and stomach after the ricin exposure, more pronounced for lung and kidney including severe bleeding. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and circular dichroism (CD) analyses revealed that although the primary structure of ricin was not altered, its secondary structures (beta-sheet and beta-turn) underwent transition upon the nsPEFs treatment.; National Natural Science Foundation of China [21277007, 21477003, 41121004]; Ministry of Science and Technology [2015CB5534, 2015DFG92040]; Ministry of Education [20130001110044]; SCI(E); PubMed; ARTICLE; yao@pku.edu.cn; zhangjue@pku.edu.cn; wjl0801@vip.163.com; 18781; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435522]
专题	环境科学与工程学院工学院
推荐引用方式 GB/T 7714	Wei, Kai,Li, Wei,Gao, Shan,et al. Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity[J]. SCIENTIFIC REPORTS,2016.
APA	Wei, Kai.,Li, Wei.,Gao, Shan.,Ji, Bin.,Zang, Yating.,...&Wang, Jinglin.(2016).Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity.SCIENTIFIC REPORTS.
MLA	Wei, Kai,et al."Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity".SCIENTIFIC REPORTS (2016).