Subcellular mechanism of Escherichia coli inactivation during   electrochemical disinfection with boron-doped diamond anode: A   comparative study of three electrolytes

doi:10.1016/j.watres.2015.07.035

CORC > 北京大学 > 环境科学与工程学院

	Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes
	Long, Yujiao ; Ni, Jinren ; Wang, Zuhui
刊名	WATER RESEARCH
	2015
关键词	Bacterial inactivation Subcellular mechanism Cell damage Membrane potential Membrane protein Intracellular enzyme VISIBLE-LIGHT PHOTOCATALYTIC DISINFECTION MEMBRANE-PERMEABILITY WATER DISINFECTION HYDROXYL RADICALS ATP SYNTHESIS CHLORINATION ELECTRODES OZONATION BACTERIA
DOI	10.1016/j.watres.2015.07.035
英文摘要	Although the identification of effective oxidant species has been extensively studied, yet the subcellular mechanism of bacterial inactivation has never been clearly elucidated in electrochemical disinfection processes. In this study, subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection was revealed in terms of comprehensive factors such as cell morphology, total organic components, K+ leakage, membrane permeability, lipid peroxidation, membrane potential, membrane proteins, intracellular enzyme, cellular ATP level and DNA. The electrolysis was conducted with boron-doped diamond anode in three electrolytes including chloride, sulfate and phosphate. Results demonstrated that cell inactivation was mainly attributed to damage to the intracellular enzymatic systems in chloride solution. In sulfate solution, certain essential membrane proteins like the K+ ion transport systems were eliminated. Thus, the pronounced K+ leakage from cytosol resulted in gradual collapse of the membrane potential, which would hinder the subcellular localization of cell division-related proteins as well as ATP synthesis and thereby lead to the bacterial inactivation. Remarkable lipid peroxidation was observed, while the intracellular damage was negligible. In phosphate solution, the cells sequentially underwent overall destruction as a whole cell with no captured intermediate state, during which the organic components of the cells were mostly subjected to mineralization. This study provided a thorough insight into the bacterial inactivation mechanism on the subcellular level (C) 2015 Elsevier Ltd. All rights reserved.; National Natural Science Foundation of China [21261140336, 51379010]; SCI(E); EI; PubMed; ARTICLE; nijinren@iee.pku.edu.cn; 198-206; 84
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/415494]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Long, Yujiao,Ni, Jinren,Wang, Zuhui. Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes[J]. WATER RESEARCH,2015.
APA	Long, Yujiao,Ni, Jinren,&Wang, Zuhui.(2015).Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes.WATER RESEARCH.
MLA	Long, Yujiao,et al."Subcellular mechanism of Escherichia coli inactivation during electrochemical disinfection with boron-doped diamond anode: A comparative study of three electrolytes".WATER RESEARCH (2015).