Comparative genomics of three Methanocellales strains reveal novel   taxonomic and metabolic features

doi:10.1111/1758-2229.12283

CORC > 北京大学 > 城市与环境学院

	Comparative genomics of three Methanocellales strains reveal novel taxonomic and metabolic features
	Lyu, Zhe ; Lu, Yahai
刊名	ENVIRONMENTAL MICROBIOLOGY REPORTS
	2015
关键词	RICE FIELD SOIL METHANE-PRODUCING ARCHAEON METHANOSARCINA-MAZEI GO1 METHANOCOCCUS-MARIPALUDIS METHANOGENIC ARCHAEA REPRESSOR NRPR CLUSTER-I SP NOV. METHANOMASSILIICOCCUS-LUMINYENSIS METHANOCALDOCOCCUS-JANNASCHII
DOI	10.1111/1758-2229.12283
英文摘要	Methanocellales represents a new order of methanogens, which is widespread in environments and plays specifically the important role in methane emissions from paddy fields. To gain more insights into Methanocellales, comparative genomic studies were performed among three Methanocellales strains through the same annotation pipeline. Genetic relationships among strains revealed by genome alignment, pan-genome reconstruction and comparison of amino average identity suggest that they should be classified in different genera. In addition, multiple copies of cell cycle regulator proteins were identified for the first time in Archaea. Core metabolisms were reconstructed, predicting certain unique and novel features for Methanocellales, including a set of methanogenesis genes potentially organized toward specialization in utilizing low concentrations of H2, a new route of disulfide reduction catalysed by a disulfide-reducing hydrogenase (Drh) complex phylogenetically related to sulfate-reducing prokaryotes, an oxidative tricarboxylic acid (TCA) cycle, a sophisticated nitrogen uptake and regulation system as well as a versatile sulfur utilization system. These core metabolisms are largely conserved among the three strains, but differences in gene copy number and metabolic diversity are evident. The present study thus adds new dimensions to the unique ecophysiology of Methanocellales and offers a road map for further experimental characterization of this methanogen lineage.; National Basic Research Program of China [2011CB100505]; National Natural Science Foundation of China [41130527]; SCI(E); ARTICLE; luyh@pku.edu.cn; 3; 526-537; 7
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/419780]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Lyu, Zhe,Lu, Yahai. Comparative genomics of three Methanocellales strains reveal novel taxonomic and metabolic features[J]. ENVIRONMENTAL MICROBIOLOGY REPORTS,2015.
APA	Lyu, Zhe,&Lu, Yahai.(2015).Comparative genomics of three Methanocellales strains reveal novel taxonomic and metabolic features.ENVIRONMENTAL MICROBIOLOGY REPORTS.
MLA	Lyu, Zhe,et al."Comparative genomics of three Methanocellales strains reveal novel taxonomic and metabolic features".ENVIRONMENTAL MICROBIOLOGY REPORTS (2015).