青鳉鱼ERRα的克隆、序列分析、组织表达及其对不同EDCs暴露的响应

CORC > 北京大学 > 城市与环境学院

	青鳉鱼ERRα的克隆、序列分析、组织表达及其对不同EDCs暴露的响应; Gene cloning, sequence analysis and tissue expression of estrogen-related receptor alpha (Erralpha) in Japanese medaka and its transcriptional responses after differential EDCs exposure
	张照斌 ; 胡建英 ; 赛思翔 ; 赵砚彬 ; 黄崇 ; 田小军
刊名	环境科学
	2008
关键词	内分泌干扰物雌激素相关受体青鳉鱼实时定量PCR
英文摘要	内分泌干扰物质(EDCs)可以通过多种通道影响鱼类的生长、发育和繁殖,而雌激素相关受体(ERR)是一类目前没有引起足够重视的潜在致毒信号通道.本研究克隆了青鳉鱼(Oryzias latipes)ERRαmRNA全序列,并通过实时定量RT-PCR方法对其在不同组织中的表达和不同EDCs暴露下的响应进行了分析.发现青鳉鱼ERRα与其它脊椎动物ERRα氨基酸序列有较高的同源性,特别是DNA结合结构域(DBD)在从鱼类到哺乳类动物的进化过程中高度保守,配体结合结构域(LBD)序列与哺乳动物的LBD有66.4%~67.0%的序列相同.青鳉鱼ERRα与青鳉鱼雌激素受体ERα、ERβ和雄激素受体ARα、AR...; Endocrine disrupting chemicals (EDCs) can bind or block nuclear receptors in the body and subsequently affect growth, development and reproduction of fish. Estrogen-related receptors (ERRs), belonging to the nuclear receptor superfamily, have been implicated in diverse physiological processes in estrogen signal pathway in mammals, while little is known about them in fishes. Complete mRNA sequence of ERRalpha from medaka (Oryzias latipes) was cloned, and the sequence is similar to those of other vertebrates, especially that the DNA-binding domain (DBD) of ERRalpha is highly conserved among the vertebrates (97.4%-100% sequence identities) and the ligand-binding domain (LBD) of medaka ERRalpha is 66.4%-67.0% sequence identities with those of mammals. The DBD of medaka ERRalpha is of the same length and has high sequence identity with those of estrogen receptor (ERalpha and ERbeta) and androgen receptor (ARalpha and ARbeta) of medaka, but much difference was found between the LBD of medaka ERRalpha with those of ERalpha, ERbeta, ARalpha and ARbeta. ERRalpha gene is located in chromosome 14 and is consisted of 5 exons. The expressions of ERRalpha in different tissues and the transcriptional responses of ERRalpha in testis of medaka exposed differential EDCs were studied by quantitative real-time RT-PCR. ERRalpha is expressed at apparently high levels in gonad, brain, eye, spleen and intestine, though it was broadly expressed in tissues. Significant transcriptional difference was found between testis and ovary, implying ERRalpha would be involved in sex differentiation and gonad development in fish. After 3 weeks exposure of medaka to 200 ng/L ethynylestradiol (EE2), 200 ng/L estrone (E1), 200 ng/L diethylstilbestrol (DES), 100 microg/L atrazine (AT) and 200 ng/L 17beta-estradiol (E2), transcripts of ERRalpha were significantly decreased to 0.54, 0.56, 0.61, 0.63 and 0.65 of control (p < 0.05) in the testes, respectively. And those in the 1 microg/L tributyltin (TBT) and 1 microg/L triphenyltin (TPT) exposure groups were up-regulated to 1.34 and 1.35 folds of control (p > 0.05), respectively. These results suggested that ERRalpha would take actions in the disruption of sex differentiation and gonad development in fish by EDCs. In addition, no multiple steroid hormone-response element half-sites was found in medaka, which were reported in the upstream of ERRalpha gene in mammals, indicating there would be different regulation patters of ERRalpha between teleost and mammal.; PubMed; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; 11; 3153-3158; 29
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/68959]
专题	城市与环境学院
推荐引用方式 GB/T 7714	张照斌,胡建英,赛思翔,等. 青鳉鱼ERRα的克隆、序列分析、组织表达及其对不同EDCs暴露的响应, Gene cloning, sequence analysis and tissue expression of estrogen-related receptor alpha (Erralpha) in Japanese medaka and its transcriptional responses after differential EDCs exposure[J]. 环境科学,2008.
APA	张照斌,胡建英,赛思翔,赵砚彬,黄崇,&田小军.(2008).青鳉鱼ERRα的克隆、序列分析、组织表达及其对不同EDCs暴露的响应.环境科学.
MLA	张照斌,et al."青鳉鱼ERRα的克隆、序列分析、组织表达及其对不同EDCs暴露的响应".环境科学 (2008).