Construction and Quality Analysis of Transgenic Rehmannia glutinosa   Containing TMV and CMV Coat Protein

doi:10.3390/molecules21091134

CORC > 北京大学 > 生命科学学院

	Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein
	Teng, Zhongqiu ; Shen, Ye ; Li, Jing ; Lin, Zhongping ; Chen, Min ; Wang, Min ; Li, Man ; Dong, Hongran ; Huang, Luqi
刊名	MOLECULES
	2016
关键词	virus resistant Rehmannia glutinosa transgenic plant medical quality biosafety TMV & POTATO-VIRUS-X SUBSTANTIAL EQUIVALENCE ENGINEERING RESISTANCE MEDIATED RESISTANCE MASS-SPECTROMETRY PLANTS GENE INFECTION TOBACCO PERFORMANCE CMV
DOI	10.3390/molecules21091134
英文摘要	Plant viruses, especially tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) are serious threats to Rehmannia glutinosa which is a top grade herb in China. In the present study, TMV- and CMV-resistant Rehmannia glutinosa Libosch. plants were constructed by transforming the protein (CP) genes of TMV and CMV into Rehmannia glutinosa via a modified procedure of Agrobacterium tumefaciens-mediated transformation. Integration and expression of TMV CP and CMV CP transgenes in 2 lines, LBA-1 and LBA-2, were confirmed by PCR, Southern blot and RT-PCR. Both LBA-1 and LBA-2 were resistant to infection of homologous TMV and CMV strains. The quality of transgenic Rehmanniae Radix was evaluated based on fingerprint analysis and components quantitative analysis comparing with control root tubes. These results showed that chemical composition of transgenic Rehmanniae Radix were similar to non-transgenic ones, which demonstrated that the medical quality and biosafety of transgenic Rehmanniae Radix were equivalent to non-transgenic material when consumed as traditional Chinese medicinal (TCM).; National Science Fund for Distinguished Young Scholars [81325023]; China Postdoctoral Science Foundation [2014M551005]; State Administration of Traditional Chinese Medicine, Chinese Medicine Scientific Research Projects [201107011]; SCI(E); PubMed; ARTICLE; zhongqiuteng@163.com; shenye70@hotmail.com; justinworking@126.com; linzp@pku.edu.cn; cm315keke@163.com; wangmincacms@sohu.com; liman887@163.com; dhyopp@126.com; huangluqi01@126.com; 9; 21
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492923]
专题	生命科学学院
推荐引用方式 GB/T 7714	Teng, Zhongqiu,Shen, Ye,Li, Jing,et al. Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein[J]. MOLECULES,2016.
APA	Teng, Zhongqiu.,Shen, Ye.,Li, Jing.,Lin, Zhongping.,Chen, Min.,...&Huang, Luqi.(2016).Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein.MOLECULES.
MLA	Teng, Zhongqiu,et al."Construction and Quality Analysis of Transgenic Rehmannia glutinosa Containing TMV and CMV Coat Protein".MOLECULES (2016).