Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively   Regulating DELLA Protein Abundance in Darkness

doi:10.1016/j.molp.2014.12.017

CORC > 北京大学 > 生命科学学院

	Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively Regulating DELLA Protein Abundance in Darkness
	Li, Kunlun ; Gao, Zhaoxu ; He, Hang ; Terzaghi, William ; Fan, Liu-Min ; Deng, Xing Wang ; Chen, Haodong
刊名	MOLECULAR PLANT
	2015
关键词	DET1 DELLA gibberellic acid (GA) photomorphogenesis Arabidopsis LIGHT CONTROL GIBBERELLIN RESPONSES TRANSCRIPTION FACTOR SEEDLING DEVELOPMENT RECEPTOR GID1 GENE EXPRESSION PATHWAY DESTABILIZATION RECOGNITION
DOI	10.1016/j.molp.2014.12.017
英文摘要	Arabidopsis De-etiolated 1 (DET1) is one of the key repressors that maintain the etiolated state of seedlings in darkness. The plant hormone gibberellic acid (GA) also participates in this process, and plants deficient in GA synthesis or signaling show a partially de-etiolated phenotype in darkness. However, how DET1 and the GA pathwaywork in concert in repressing photomorphogenesis remains largely unknown. In this study, we found that the abundance of DELLA proteins in det1-1 was increased in comparison with that in the wild-type plants. Mutation in DET1 changed the sensitivity of hypocotyl elongation of mutant seedlings to GA and paclobutrazol (PAC), an inhibitor of GA synthesis. However, we did not find obvious differences between det1-1 and wild-type plants with regard to the bioactive GA content or the GA signaling upstream of DELLAs. Genetic data showed that removal of several DELLA proteins suppressed the det1-1 mutant phenotype more obviously than GA treatment, indicating that DET1 can regulate DELLA proteins via some other mechanisms. In addition, a large-scale transcriptomic analysis revealed that DET1 and DELLAs play antagonistic roles in regulating expression of photosynthetic and cell elongation-related genes in etiolated seedlings. Taken together, our results show that DET1 represses photomorphogenesis in darkness in part by reducing the abundance of DELLA proteins.; Peking-Tsinghua Center for Life Sciences, and State Key Laboratory of Protein and Plant Gene Research,the National Program on Key Basic Research Project of China (973 Program),grants to X.W.D. from the National Natural Science Foundation of China,State Key Laboratory of Protein and Plant Gene Research,the Ministry of Agriculture of China (948 Program),the National High Technology Research and Development Program of China (863 Program),supported by grants to H.C. from the National Natural Science Foundation of China,the National Program on Key Basic Research Project of China (973 Program); SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); ARTICLE; xingwang.deng@yale.edu; chenhaodong@pku.edu.cn; 4; 622-630; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492297]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Kunlun,Gao, Zhaoxu,He, Hang,et al. Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively Regulating DELLA Protein Abundance in Darkness[J]. MOLECULAR PLANT,2015.
APA	Li, Kunlun.,Gao, Zhaoxu.,He, Hang.,Terzaghi, William.,Fan, Liu-Min.,...&Chen, Haodong.(2015).Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively Regulating DELLA Protein Abundance in Darkness.MOLECULAR PLANT.
MLA	Li, Kunlun,et al."Arabidopsis DET1 Represses Photomorphogenesis in Part by Negatively Regulating DELLA Protein Abundance in Darkness".MOLECULAR PLANT (2015).