Enhanced optogenetic cell ablation in C. elegans using membrane-targeted mini SOG; Enhanced optogenetic cell ablation in C. elegans using membrane-targeted mini SOG | |
Suhong Xu ; Wei Dong | |
2016 | |
关键词 | ablation targeted singlet neuronal illumination killing classic correlative Arabidopsis expand ablation targeted singlet neuronal illumination killing classic correlative Arabidopsis expand |
英文摘要 | Cell ablation is a classic approach to understand cell and tissue function in development or neuronal function in vivo. mini singlet oxygen generator(mini SOG), engineered from the LOV domain of Arabidopsis phototropin 2, was originally developed for correlative light and electron microscopy(Shu et al 2011 PLOS Biol). After illumination with blue light, mini SOG generates the reactive oxygen species singlet oxygen(1O2). Illumination of neurons expressing mini SOG targeted to the outer mitochondrial membrane(mito-mini SOG) causes neuronal death by a non-apoptotic mechanism, allowing photo-inducible cell ablation(Qi et al 2012 PNAS). Since its development as a cell killing tool, mini SOG has been used in a variety of ablation experiments, using blue light exposures of 0.5-1.5 h. However, extended blue light exposure itself can be deleterious to C. elegans. We found that membrane targeted mini SOG allows highly efficient ablation of multiple cell types, including neurons, muscles, and the epidermis(Xu and Chisho...; Cell ablation is a classic approach to understand cell and tissue function in development or neuronal function in vivo. mini singlet oxygen generator(mini SOG), engineered from the LOV domain of Arabidopsis phototropin 2, was originally developed for correlative light and electron microscopy(Shu et al 2011 PLOS Biol). After illumination with blue light, mini SOG generates the reactive oxygen species singlet oxygen(1O2). Illumination of neurons expressing mini SOG targeted to the outer mitochondrial membran; 中国生物物理学会、北京细胞生物学会、生物大分子国家重点实验室; 1 |
语种 | 英语 |
出处 | 第七届亚太地区秀丽线虫学术研讨会 |
内容类型 | 其他 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/479971] |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | Suhong Xu,Wei Dong. Enhanced optogenetic cell ablation in C. elegans using membrane-targeted mini SOG, Enhanced optogenetic cell ablation in C. elegans using membrane-targeted mini SOG. 2016-01-01. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论