营养缺乏和碱性环境对粪肠球菌侵入人工微管系统的影响; The effects of environmental changes on E.faecalis growth and penetration into microtubes in a microfluid chip | |
孙晓强 ; 王淑静 ; 罗春雄 ; 侯本祥 | |
刊名 | 实用口腔医学杂志
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2017 | |
关键词 | 牙本质小管 粪肠球菌 微流芯片 持续性根尖周炎 Dentinal tubules Enterococcus faecalis Microfluidics Persistent apical periodontitis |
DOI | 10.3969/j.issn.1001-3733.2017.01.011 |
英文摘要 | 目的:研究不同环境因素对粪肠球菌侵入人工微管系统能力的影响和微流芯片的适用性.方法:采用软光刻技术制作微流芯片,观察细菌在牛脑心浸出液(BHI)、PBS和pH 10的情况下在微流芯片中的生长情况,测定和比较细菌在相应微管内的最大侵入深度.结果:粪肠球菌在营养缺乏和碱性环境下生长速度明显下降,侵入小管径微管的深度显著下降(P<0.01).结论:营养缺乏和碱性环境可显著降低粪肠球菌侵入微管系统的能力.微流芯片具有高通量、平行对比和可观察性好等特点,有望为今后相关研究提供标准化的实验平台.; Objective:To evaluate the effects of different growth conditions on E.faecalis growth in the microfluid chip and the penetration of E.faecalis into microtubes.Methods:Four units on the chip were randomly selected as control,BHI,nutrient-Tdeprived (PBS) and pH 10 groups.The growth of E.faecalis was monitored by microscope for a period of 72 h after the suspension of E.faecalis had been added into the chip.Results:The microscopic analysis showed a distinct variation in the growth rate and morphological feature under different experimental conditions.he depth of bacterial penetration was significantly greater in BHI group.Conclusion:This study demonstrated that environmental changes can significantly influence the growth and penetration of E.faecalis into the micro tubes.; 国家自然科学基金; 北京市医院管理局临床医学发展专项经费资助; 中国科学引文数据库(CSCD); 1; 49-53; 33 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/477759] ![]() |
专题 | 生命科学学院 |
推荐引用方式 GB/T 7714 | 孙晓强,王淑静,罗春雄,等. 营养缺乏和碱性环境对粪肠球菌侵入人工微管系统的影响, The effects of environmental changes on E.faecalis growth and penetration into microtubes in a microfluid chip[J]. 实用口腔医学杂志,2017. |
APA | 孙晓强,王淑静,罗春雄,&侯本祥.(2017).营养缺乏和碱性环境对粪肠球菌侵入人工微管系统的影响.实用口腔医学杂志. |
MLA | 孙晓强,et al."营养缺乏和碱性环境对粪肠球菌侵入人工微管系统的影响".实用口腔医学杂志 (2017). |
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