Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by   Nmd3, Lsg1, Tif6 and Reh1

doi:10.1038/nsmb.3364

CORC > 北京大学 > 生命科学学院

	Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1
	Ma, Chengying ; Wu, Shan ; Li, Ningning ; Chen, Yan ; Yan, Kaige ; Li, Zhifei ; Zheng, Lvqin ; Lei, Jianlin ; Woolford, John L., Jr. ; Gao, Ning
刊名	NATURE STRUCTURAL & MOLECULAR BIOLOGY
	2017
关键词	SHWACHMAN-DIAMOND SYNDROME NUCLEAR EXPORT RECEPTOR 60S SUBUNIT MATURATION INITIATION-FACTOR 6 SACCHAROMYCES-CEREVISIAE EUKARYOTIC RIBOSOME FUNCTIONAL DOMAINS BACTERIAL RIBOSOME CRYSTAL-STRUCTURE ADAPTER PROTEIN
DOI	10.1038/nsmb.3364
英文摘要	A key step in ribosome biogenesis is the nuclear export of pre-ribosomal particles. Nmd3, a highly conserved protein in eukaryotes, is a specific adaptor required for the export of pre-60S particles. Here we used cryo-electron microscopy (cryo-EM) to characterize Saccharomyces cerevisiae pre-60S particles purified with epitope-tagged Nmd3. Our structural analysis indicates that these particles belong to a specific late stage of cytoplasmic pre-60S maturation in which ribosomal proteins uL16, uL10, uL11, eL40 and eL41 are deficient, but ribosome assembly factors Nmd3, Lsg1, Tif6 and Reh1 are present. Nmd3 and Lsg1 are located near the peptidyl-transferase center (PTC). In particular, Nmd3 recognizes the PTC in its near-mature conformation. In contrast, Reh1 is anchored to the exit of the polypeptide tunnel, with its C terminus inserted into the tunnel. These findings pinpoint a structural checkpoint role for Nmd3 in PTC assembly, and provide information about functional and mechanistic roles of these assembly factors in the maturation of the 60S ribosomal subunit.; National Natural Science Foundation of China [31422016, 31470722, 31630087]; Ministry of Science and Technology of China [2016YFA0500700, 2013CB910404]; NIH [R01GM028301]; Peking-Tsinghua Center for Life Sciences; SCI(E); ARTICLE; 3; 214-+; 24
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474955]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ma, Chengying,Wu, Shan,Li, Ningning,et al. Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2017.
APA	Ma, Chengying.,Wu, Shan.,Li, Ningning.,Chen, Yan.,Yan, Kaige.,...&Gao, Ning.(2017).Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1.NATURE STRUCTURAL & MOLECULAR BIOLOGY.
MLA	Ma, Chengying,et al."Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1".NATURE STRUCTURAL & MOLECULAR BIOLOGY (2017).