Interaction of gold and silver nanoparticles with human plasma: Analysis   of protein corona reveals specific binding patterns

doi:10.1016/j.colsurfb.2017.01.037

CORC > 北京大学 > 生命科学学院

	Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns
	Lai, Wenjia ; Wang, Qingsong ; Li, Lumeng ; Hu, Zhiyuan ; Chen, Jiankui ; Fang, Qiaojun
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2017
关键词	Silver nanoparticle Gold nanoparticle Human plasma Corona Surface properties COMPLEMENT ACTIVATION SERUM-ALBUMIN GENE ONTOLOGY SIZE SURFACES ADSORPTION FIBRINOGEN INTERFACE PROVIDE HARD
DOI	10.1016/j.colsurfb.2017.01.037
英文摘要	Determining how nanomaterials interact with plasma will assist in understanding their effects on the biological system. This work presents a systematic study of the protein corona formed from human plasma on 20 nm silver and gold nanoparticles with three different surface modifications, including positive and negative surface charges. The results show that all nanoparticles, even those with positive surface modifications, acquire negative charges after interacting with plasma. Approximately 300 proteins are identified on the coronas, while 99 are commonly found on each nanomaterial. The 20 most abundant proteins account for over 80% of the total proteins abundance. Remarkably, the surface charge and core of the nanoparticles, as well as the isoelectric point of the plasma proteins, are found to play significant roles in determining the nanoparticle coronas. Albumin and globulins are present at levels of less than 2% on these nanoparticle coronas. Fibrinogen, which presents in the plasma but not in the serum, preferably binds to negatively charged gold nanoparticles. These observations demonstrate the specific plasma protein binding pattern of silver and gold nanoparticles, as well as the importance of the surface charge and core in determining the protein corona compositions. The potential downstream biological impacts of the corona proteins were also investigated. (C) 2017 Elsevier B.V. All rights reserved.; Chinese Academy of Sciences [Y4362911ZX, XDA09040300]; SCI(E); ARTICLE; 317-325; 152
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474336]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lai, Wenjia,Wang, Qingsong,Li, Lumeng,et al. Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2017.
APA	Lai, Wenjia,Wang, Qingsong,Li, Lumeng,Hu, Zhiyuan,Chen, Jiankui,&Fang, Qiaojun.(2017).Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns.COLLOIDS AND SURFACES B-BIOINTERFACES.
MLA	Lai, Wenjia,et al."Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns".COLLOIDS AND SURFACES B-BIOINTERFACES (2017).