Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis   in Mammalian Cells

doi:10.1016/j.jmb.2017.01.016

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	Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells
	Li, Miao ; Dong, Qiang ; Zhu, Bing
刊名	JOURNAL OF MOLECULAR BIOLOGY
	2017
关键词	MITOTIC CHROMOSOME CONDENSATION VARIANT H3.3
DOI	10.1016/j.jmb.2017.01.016
英文摘要	Serine 31 of histone H3.3 is phosphorylated during mitosis in mammalian cells and has been shown to influence chromosome segregation. We established a screening system to identify candidate kinase(s) phosphorylating H3.3S31 by using siRNA library targeting 720 human kinases, and we confirmed the results with an independent kinase inhibitor screen. Aurora kinase B was the best candidate hit in both screens. Moreover, the kinase activity of Aurora B on H3.3S31 was biochemically confirmed with nucleosomal substrates in vitro. (C) 2017 Elsevier Ltd. All rights reserved.; China Natural Science Foundation [31530037]; Chinese Ministry of Science and Technology [2015CB856200]; Chinese Academy of Sciences [XDB08010103]; Howard Hughes Medical Institute International Early Career Scientist Program; SCI(E); ARTICLE; 13; 2042-2045; 429
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472596]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Miao,Dong, Qiang,Zhu, Bing. Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells[J]. JOURNAL OF MOLECULAR BIOLOGY,2017.
APA	Li, Miao,Dong, Qiang,&Zhu, Bing.(2017).Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells.JOURNAL OF MOLECULAR BIOLOGY.
MLA	Li, Miao,et al."Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells".JOURNAL OF MOLECULAR BIOLOGY (2017).