Conformational Landscape of the p28-Bound Human Proteasome Regulatory   Particle

doi:10.1016/j.molcel.2017.06.007

CORC > 北京大学 > 生命科学学院

	Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle
	Lu, Ying ; Wu, Jiayi ; Dong, Yuanchen ; Chen, Shuobing ; Sun, Shuangwu ; Ma, Yong-Bei ; Ouyang, Qi ; Finley, Daniel ; Kirschner, Marc W. ; Mao, Youdong
刊名	MOLECULAR CELL
	2017
关键词	HUMAN 26S PROTEASOME EM STRUCTURE DETERMINATION CRYO-EM METHANOCALDOCOCCUS-JANNASCHII MOLECULAR ARCHITECTURE ELECTRON-MICROSCOPY STRUCTURAL BASIS S PROTEASOME DEGRADATION CHAPERONE
DOI	10.1016/j.molcel.2017.06.007
英文摘要	The proteasome holoenzyme is activated by its regulatory particle (RP) consisting of two subcomplexes, the lid and the base. A key event in base assembly is the formation of a heterohexameric ring of AAA-ATPases, which is guided by at least four RP assembly chaperones in mammals: PAAF1, p28/gankyrin, p27/PSMD9, and S5b. Using cryogenic electron microscopy, we analyzed the non-AAA structure of the p28-bound human RP at 4.5 angstrom resolution and determined seven distinct conformations of the Rpn1-p28-AAA subcomplex within the p28-bound RP at subnanometer resolutions. Remarkably, the p28bound AAA ring does not form a channel in the free RP and spontaneously samples multiple "open" and "closed" topologies at the Rpt2-Rpt6 and Rpt3-Rpt4 interfaces. Our analysis suggests that p28 assists the proteolytic core particle to select a specific conformation of the ATPase ring for RP engagement and is released in a shoehorn-like fashion in the last step of the chaperone-mediated proteasome assembly.; NIGMS [GM026875]; Thousand Talents Plan of China; Intel academic grant; Peking-Tsinghua Center for Life Science at Peking University; National Natural Science Foundation of China [91530321]; NIH [R01 GM095526]; National Science Foundation under NSF [1541959]; NIH, Center for HIV/AIDS Vaccine Immunology and Immunogen Design (CHAVI-ID) [AI100645]; SCI(E); ARTICLE; 2; 322-+; 67
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472066]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lu, Ying,Wu, Jiayi,Dong, Yuanchen,et al. Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle[J]. MOLECULAR CELL,2017.
APA	Lu, Ying.,Wu, Jiayi.,Dong, Yuanchen.,Chen, Shuobing.,Sun, Shuangwu.,...&Mao, Youdong.(2017).Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.MOLECULAR CELL.
MLA	Lu, Ying,et al."Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle".MOLECULAR CELL (2017).