Real time determination of bacterial in vivo ribosome translation   elongation speed based on LacZ alpha complementation system

doi:10.1093/nar/gkw698

CORC > 北京大学 > 生命科学学院

	Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZ alpha complementation system
	Zhu, Manlu ; Dai, Xiongfeng ; Wang, Yi-Ping
刊名	NUCLEIC ACIDS RESEARCH
	2016
关键词	COLI B-R ESCHERICHIA-COLI BETA-GALACTOSIDASE CHAIN-ELONGATION GENE-EXPRESSION CELL-GROWTH ACID MUTANTS RATES
DOI	10.1093/nar/gkw698
英文摘要	Bacterial growth significantly depends on protein synthesis catalyzed by ribosome. Ribosome translation elongation speed is a key factor determining the bacterial protein synthesis rate. However, existing methods for determining translation elongation speed have limited applications. Here we developed a simple and convenient method for measuring bacterial translation elongation speed based on LacZ alpha complementation system. It enables the measurement of in vivo translation elongation speed of different individual genes. Tests related to ribosome translation elongation speed under various growth perturbations including different nutrient conditions, low temperature, a low-speed ribosome mutant, and fusidic acid treatment, were performed to quantitatively validate this method. Using this approach, we further found that nutrient starvation caused a remarkable slow-down of ribosome translation of Es-cherichia coli (E. coli). We also studied the dynamic change of translation elongation speed during the process of nutrient up-shift. This method will boost the quantitative understanding of bacterial ribosome translation capacity and growth.; National Natural Science Fund (NNSF) of the People's Republic of China (NSFC) [31530081, 31270127]; NSFC for Distinguished Young Scholars [9925017]; SCI(E); ARTICLE; daixiongfeng@pku.edu.cn; wangyp@pku.edu.cn; 20; 44
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/458490]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhu, Manlu,Dai, Xiongfeng,Wang, Yi-Ping. Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZ alpha complementation system[J]. NUCLEIC ACIDS RESEARCH,2016.
APA	Zhu, Manlu,Dai, Xiongfeng,&Wang, Yi-Ping.(2016).Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZ alpha complementation system.NUCLEIC ACIDS RESEARCH.
MLA	Zhu, Manlu,et al."Real time determination of bacterial in vivo ribosome translation elongation speed based on LacZ alpha complementation system".NUCLEIC ACIDS RESEARCH (2016).