CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic   Resistance in Both Laboratory and Clinical nalB-Type Isolates of   Pseudomonas aeruginosa

doi:10.1371/journal.ppat.1005932

CORC > 北京大学 > 生命科学学院

	CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa
	Tian, Zhe-Xian ; Yi, Xue-Xian ; Cho, Anna ; O&apos ; Wang, Yi-Ping ; Gara, Fergal
刊名	PLOS PATHOGENS
	2016
关键词	ENVELOPE STRESS-RESPONSE COLI CELL-ENVELOPE ESCHERICHIA-COLI 2-COMPONENT SYSTEM MEMBRANE-PROTEINS COREGULATED GENES VIBRIO-CHOLERAE BINDING-SITES REPRESSOR REGULATOR
DOI	10.1371/journal.ppat.1005932
英文摘要	Resistance-Nodulation-Division (RND) efflux pumps are responsible for multidrug resistance in Pseudomonas aeruginosa. In this study, we demonstrate that CpxR, previously identified as a regulator of the cell envelope stress response in Escherichia coli, is directly involved in activation of expression of RND efflux pump MexAB-OprM in P. aeruginosa. A conserved CpxR binding site was identified upstream of the mexA promoter in all genome-sequenced P. aeruginosa strains. CpxR is required to enhance mexAB-oprM expression and drug resistance, in the absence of repressor MexR, in P. aeruginosa strains PA14. As defective mexR is a genetic trait associated with the clinical emergence of nalB-type multidrug resistance in P. aeruginosa during antibiotic treatment, we investigated the involvement of CpxR in regulating multidrug resistance among resistant isolates generated in the laboratory via antibiotic treatment and collected in clinical settings. CpxR is required to activate expression of mexAB-oprM and enhances drug resistance, in the absence or presence of MexR, in ofloxacin-cefsulodin-resistant isolates generated in the laboratory. Furthermore, CpxR was also important in the mexR-defective clinical isolates. The newly identified regulatory linkage between CpxR and the MexAB-OprM efflux pump highlights the presence of a complex regulatory network modulating multidrug resistance in P. aeruginosa.; National Science Foundation of China [31270127, 81071398]; State Key Laboratory of Protein and Plant Gene Research [B02]; National Science Fund for Distinguished Young Scholars [39925017]; SCI(E); PubMed; ARTICLE; tianzx@pku.edu.cn; wangyp@pku.edu.cn; 10; e1005932; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457443]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tian, Zhe-Xian,Yi, Xue-Xian,Cho, Anna,et al. CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa[J]. PLOS PATHOGENS,2016.
APA	Tian, Zhe-Xian,Yi, Xue-Xian,Cho, Anna,O&apos,Wang, Yi-Ping,&Gara, Fergal.(2016).CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa.PLOS PATHOGENS.
MLA	Tian, Zhe-Xian,et al."CpxR Activates MexAB-OprM Efflux Pump Expression and Enhances Antibiotic Resistance in Both Laboratory and Clinical nalB-Type Isolates of Pseudomonas aeruginosa".PLOS PATHOGENS (2016).