Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore   targeting of Mad1-Mad2

doi:10.1038/ncomms10151

CORC > 北京大学 > 生命科学学院

	Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2
	Zhou, Haining ; Wang, Tianning ; Zheng, Tao ; Teng, Junlin ; Chen, Jianguo
刊名	NATURE COMMUNICATIONS
	2016
关键词	SPINDLE-ASSEMBLY CHECKPOINT MOSAIC VARIEGATED ANEUPLOIDY MITOTIC CHECKPOINT UNATTACHED KINETOCHORES MICROTUBULE ATTACHMENT CHROMOSOME CONGRESSION SPECIFIES LOCALIZATION NDC80 COMPLEX HUMAN-CELLS MAD2
DOI	10.1038/ncomms10151
英文摘要	The spindle assembly checkpoint (SAC) arrests cells in mitosis by sensing unattached kinetochores, until all chromosomes are bi-oriented by spindle microtubules. Kinetochore accumulation of the SAC component Mad1-Mad2 is crucial for SAC activation. However, the mechanism by which Mad1-Mad2 accumulation at kinetochores is regulated is not clear. Here we find that Cep57 is localized to kinetochores in human cells, and binds to Mis12, a KMN (KNL1/Mis12 complex/Ndc80 complex) network component. Cep57 also interacts with Mad1, and depletion of Cep57 results in decreased kinetochore localization of Mad1-Mad2, reduced SAC signalling and increased chromosome segregation errors. We also show that the microtubule-binding activity of Cep57 is involved in the timely removal of Mad1 from kinetochores. Thus, these findings reveal that the KMN network-binding protein Cep57 is a mitotic kinetochore component, and demonstrate the functional connection between the KMN network and the SAC.; National Natural Science Foundation of China [31371349, 31171283]; SCI(E); PubMed; ARTICLE; junlinteng@pku.edu.cn; chenjg@pku.edu.cn; 10151; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435458]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhou, Haining,Wang, Tianning,Zheng, Tao,et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2[J]. NATURE COMMUNICATIONS,2016.
APA	Zhou, Haining,Wang, Tianning,Zheng, Tao,Teng, Junlin,&Chen, Jianguo.(2016).Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.NATURE COMMUNICATIONS.
MLA	Zhou, Haining,et al."Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2".NATURE COMMUNICATIONS (2016).