Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in   Microcystis-Dominated Community

doi:10.3389/fmicb.2016.00056

CORC > 北京大学 > 生命科学学院

	Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community
	Xie, Meili ; Ren, Minglei ; Yang, Chen ; Yi, Haisi ; Li, Zhe ; Li, Tao ; Zhao, Jindong
刊名	FRONTIERS IN MICROBIOLOGY
	2016
关键词	metagenome binning Microcystis bloom symbiosis HETEROTROPHIC BACTERIA MAXIMUM-LIKELIHOOD DENSITY-ESTIMATION SEQUENCES CYANOBACTERIA AERUGINOSA LAKE LIPOPOLYSACCHARIDE ALGORITHM GENOMES
DOI	10.3389/fmicb.2016.00056
英文摘要	Microcystis bloom, a cyanobacterial mass occurrence often found in eutrophicated water bodies, is one of the most serious threats to freshwater ecosystems worldwide. In nature, Microcystis forms aggregates or colonies that contain heterotrophic bacteria. The Microcystis-bacteria colonies were persistent even when they were maintained in lab culture for a long period. The relationship between Microcystis and the associated bacteria was investigated by a metagenomic approach in this study. We developed a visualization -guided method of binning for genome assembly after total colony DNA sequencing. We found that the method was effective in grouping sequences and it did not require reference genome sequence. Individual genomes of the colony bacteria were obtained and they provided valuable insights into microbial community structures. Analysis of metabolic pathways based on these genomes revealed that while all heterotrophic bacteria were dependent upon Microcystis for carbon and energy, Vitamin B12 biosynthesis, which is required for growth by Microcystis, was accomplished in a cooperative fashion among the bacteria. Our analysis also suggests that individual bacteria in the colony community contributed a complete pathway for degradation of benzoate, which is inhibitory to the cyanobacterial growth, and its ecological implication for Microcystis bloom is discussed.; National Natural Science Foundation of China [31000113]; National High-Technology Research and Development Program of China (863 Program) [2009AA02Z30]; Autonomous Projects of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ31, 2011FBZ32]; SCI(E); PubMed; ARTICLE; lizhe@ibcas.ac.cn; litao@ihb.ac.cn; 56; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434975]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xie, Meili,Ren, Minglei,Yang, Chen,et al. Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community[J]. FRONTIERS IN MICROBIOLOGY,2016.
APA	Xie, Meili.,Ren, Minglei.,Yang, Chen.,Yi, Haisi.,Li, Zhe.,...&Zhao, Jindong.(2016).Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community.FRONTIERS IN MICROBIOLOGY.
MLA	Xie, Meili,et al."Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community".FRONTIERS IN MICROBIOLOGY (2016).