DNA replication initiator Cdc6 also regulates ribosomal DNA   transcription initiation

doi:10.1242/jcs.178723

CORC > 北京大学 > 生命科学学院

	DNA replication initiator Cdc6 also regulates ribosomal DNA transcription initiation
	Huang, Shijiao ; Xu, Xiaowei ; Wang, Guopeng ; Lu, Guoliang ; Xie, Wenbing ; Tao, Wei ; Zhang, Hongyin ; Jiang, Qing ; Zhang, Chuanmao
刊名	JOURNAL OF CELL SCIENCE
	2016
关键词	Cdc6 RNA polymerase I RRN3 Cell cycle Nucleophosmin rDNA transcription RNA-POLYMERASE-I TATA-BINDING PROTEIN MCM PROTEINS HUMAN-CELLS FACTOR SL1 PREREPLICATION COMPLEXES RDNA TRANSCRIPTION BUDDING YEAST FACTOR UBF CHROMATIN
DOI	10.1242/jcs.178723
英文摘要	RNA-polymerase-I-dependent ribosomal DNA (rDNA) transcription is fundamental to rRNA processing, ribosome assembly and protein synthesis. However, how this process is initiated during the cell cycle is not fully understood. By performing a proteomic analysis of transcription factors that bind RNA polymerase I during rDNA transcription initiation, we identified that the DNA replication initiator Cdc6 interacts with RNA polymerase I and its co-factors, and promotes rDNA transcription in G1 phase in an ATPase-activity-dependent manner. We further showed that Cdc6 is targeted to the nucleolus during late mitosis and G1 phase in a manner that is dependent on B23 (also known as nucleophosmin, NPM1), and preferentially binds to the rDNA promoter through its ATP-binding domain. Overexpression of Cdc6 increases rDNA transcription, whereas knockdown of Cdc6 results in a decreased association of both RNA polymerase I and the RNA polymerase I transcription factor RRN3 with rDNA, and a reduction of rDNA transcription. Furthermore, depletion of Cdc6 impairs the interaction between RRN3 and RNA polymerase I. Taken together, our data demonstrate that Cdc6 also serves as a regulator of rDNA transcription initiation, and indicate a mechanism by which initiation of rDNA transcription and DNA replication can be coordinated in cells.; National Natural Science Foundation of China (NSFC) [31430051, 31520103906, 31371365]; State Key Basic Research and Development Plan of the Ministry of Science and Technology of China [2014CB138402]; SCI(E); PubMed; ARTICLE; zhangcm@pku.edu.cn; 7; 1429-1440; 129
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434958]
专题	生命科学学院
推荐引用方式 GB/T 7714	Huang, Shijiao,Xu, Xiaowei,Wang, Guopeng,et al. DNA replication initiator Cdc6 also regulates ribosomal DNA transcription initiation[J]. JOURNAL OF CELL SCIENCE,2016.
APA	Huang, Shijiao.,Xu, Xiaowei.,Wang, Guopeng.,Lu, Guoliang.,Xie, Wenbing.,...&Zhang, Chuanmao.(2016).DNA replication initiator Cdc6 also regulates ribosomal DNA transcription initiation.JOURNAL OF CELL SCIENCE.
MLA	Huang, Shijiao,et al."DNA replication initiator Cdc6 also regulates ribosomal DNA transcription initiation".JOURNAL OF CELL SCIENCE (2016).