A Land Plant-Specific Transcription Factor Directly Enhances   Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent   RNA Polymerase II

doi:10.1105/tpc.16.00100

CORC > 北京大学 > 生命科学学院

	A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II
	Wang, Ying ; Qu, Jie ; Ji, Shaoyi ; Wallace, Andrew J. ; Wu, Jian ; Li, Yi ; Gopalan, Venkat ; Ding, Biao
刊名	PLANT CELL
	2016
关键词	5S RIBOSOMAL-RNA HEPATITIS-DELTA VIRUS VIROID-HOST INTERACTIONS ZINC FINGERS ARABIDOPSIS-THALIANA PROTEIN VIRP1 REPLICATION COMPLEX TFIIIA ASSOCIATION
DOI	10.1105/tpc.16.00100
英文摘要	Some DNA-dependent RNA polymerases (DdRPs) possess RNA-dependent RNA polymerase activity, as was first discovered in the replication of Potato spindle tuber viroid (PSTVd) RNA genome in tomato (Solanum lycopersicum). Recent studies revealed that this activity in bacteria and mammals is important for transcriptional and posttranscriptional regulatory mechanisms. Here, we used PSTVd as a model to uncover auxiliary factors essential for RNA-templated transcription by DdRP. PSTVd replication in the nucleoplasm generates (-)-PSTVd intermediates and (+)-PSTVd copies. We found that the Nicotiana benthamiana canonical 9-zinc finger (ZF) Transcription Factor IIIA (TFIIIA-9ZF) as well as its variant TFIIIA-7ZF interacted with (+)-PSTVd, but only TFIIIA-7ZF interacted with (-)-PSTVd. Suppression of TFIIIA-7ZF reduced PSTVd replication, and overexpression of TFIIIA-7ZF enhanced PSTVd replication in planta. Consistent with the locale of PSTVd replication, TFIIIA-7ZF was found in the nucleoplasm and nucleolus, in contrast to the strictly nucleolar localization of TFIIIA-9ZF. Footprinting assays revealed that only TFIIIA-7ZF bound to a region of PSTVd critical for initiating transcription. Furthermore, TFIIIA-7ZF strongly enhanced the in vitro transcription of circular (+)-PSTVd by partially purified Pol II. Together, our results identify TFIIIA-7ZF as a dedicated cellular transcription factor that acts in DdRP-catalyzed RNA-templated transcription, highlighting both the extraordinary evolutionary adaptation of viroids and the potential of DdRPs for a broader role in cellular processes.; U.S. National Science Foundation [IOS-1051655, IOS-1354635, MCB-0843543]; Ohio State University Center for RNA Biology; Natural Science Foundation of China (NSFC) [31420103904]; Excellence in PMBB Graduate Fellowship (OSU); NSF Research Experience for Undergraduates on-site grant [DBI-1062144]; Pelotonia Fellowship (OSU); SCI(E); PubMed; ARTICLE; wang.974@osu.edu; 5; 1094-1107; 28
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433972]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Ying,Qu, Jie,Ji, Shaoyi,et al. A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II[J]. PLANT CELL,2016.
APA	Wang, Ying.,Qu, Jie.,Ji, Shaoyi.,Wallace, Andrew J..,Wu, Jian.,...&Ding, Biao.(2016).A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II.PLANT CELL.
MLA	Wang, Ying,et al."A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II".PLANT CELL (2016).