Noncoding Transcription Is a Driving Force for Nucleosome Instability in   spt16 Mutant Cells

doi:10.1128/MCB.00152-16

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	Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant Cells
	Feng, Jianxun ; Gan, Haiyun ; Eaton, Matthew L. ; Zhou, Hui ; Li, Shuqi ; Belsky, Jason A. ; MacAlpine, David M. ; Zhang, Zhiguo ; Li, Qing
刊名	MOLECULAR AND CELLULAR BIOLOGY
	2016
关键词	HISTONE H3 EXCHANGE SACCHAROMYCES-CEREVISIAE DNA-REPLICATION CRYPTIC TRANSCRIPTION RNA-POLYMERASE GENOME-WIDE BIDIRECTIONAL PROMOTERS ANTISENSE TRANSCRIPTION EUKARYOTIC GENOME YEAST
DOI	10.1128/MCB.00152-16
英文摘要	FACT (facilitates chromatin transcription) consists of two essential subunits, Spt16 and Pob3, and functions as a histone chaperone. Mutation of spt16 results in a global loss of nucleosomes as well as aberrant transcription. Here, we show that the majority of nucleosome changes upon Spt16 depletion are alterations in nucleosome fuzziness and position shift. Most nucleosomal changes are suppressed by the inhibition of RNA polymerase II (Pol II) activity. Surprisingly, a small subgroup of nucleosome changes is resistant to transcriptional inhibition. Notably, Spt16 and distinct histone modifications are enriched at this subgroup of nucleosomes. We also report 1,037 Spt16-suppressed noncoding transcripts (SNTs) and found that the SNT start sites are enriched with the subgroup of nucleosomes resistant to Pol II inhibition. Finally, the nucleosomes at genes overlapping SNTs are more susceptible to changes upon Spt16 depletion than those without SNTs. Taken together, our results support a model in which Spt16 has a role in maintaining local nucleosome stability to inhibit initiation of SNT transcription, which once initiated drives additional nucleosome loss upon Spt16 depletion.; National Institutes of Health (NIH) [GM81838]; HHS \ National Institutes of Health (NIH) [GM104097]; National Natural Science Foundation of China (NSFC) [31322017, 31370767]; SCI(E); PubMed; ARTICLE; Zhang.Zhiguo@mayo.edu; li.qing@pku.edu.cn; 13; 1856-1867; 36
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433879]
专题	生命科学学院
推荐引用方式 GB/T 7714	Feng, Jianxun,Gan, Haiyun,Eaton, Matthew L.,et al. Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant Cells[J]. MOLECULAR AND CELLULAR BIOLOGY,2016.
APA	Feng, Jianxun.,Gan, Haiyun.,Eaton, Matthew L..,Zhou, Hui.,Li, Shuqi.,...&Li, Qing.(2016).Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant Cells.MOLECULAR AND CELLULAR BIOLOGY.
MLA	Feng, Jianxun,et al."Noncoding Transcription Is a Driving Force for Nucleosome Instability in spt16 Mutant Cells".MOLECULAR AND CELLULAR BIOLOGY (2016).