AtVPS41-mediated endocytic pathway is essential for pollen tube-stigma   interaction in Arabidopsis

doi:10.1073/pnas.1602757113

CORC > 北京大学 > 生命科学学院

	AtVPS41-mediated endocytic pathway is essential for pollen tube-stigma interaction in Arabidopsis
	Hao, Lihong ; Liu, Jingjing ; Zhong, Sheng ; Gu, Hongya ; Qu, Li-Jia
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2016
关键词	VPS41 pollen tube-stigma interaction PVC-to-vacuole trafficking endocytic pathway Arabidopsis HOPS TETHERING COMPLEX VACUOLAR TRAFFICKING DOUBLE FERTILIZATION SPLIT-UBIQUITIN IN-VIVO PROTEIN FUSION GENE ENDOSOME SYSTEM
DOI	10.1073/pnas.1602757113
英文摘要	In flowering plants, extensive male-female interactions are required for successful fertilization in which various signaling cascades are involved. Prevacuolar compartments (PVC) and vacuoles are two types of subcellular compartments that terminate signal transduction by sequestrating signaling molecules in yeast and mammalian cells; however, the manner in which they might be involved in male-female interactions in plants is unknown. In this study, we identified Arabidopsis thaliana vacuolar protein sorting 41 (AtVPS41), encoded by a single-copy gene with sequence similarity to yeast Vps41p, as a new factor controlling pollen tube-stigma interaction. Loss of AtVPS41 function disrupted penetration of pollen tubes into the transmitting tissue and thus led to failed male transmission. In the pollen tubes, AtVPS41 protein is associated with PVCs and the tonoplast. We demonstrate that AtVPS41 is required for the late stage of the endocytic pathway (i.e., endomembrane trafficking from PVCs to vacuoles) because internalization of cell-surface molecules was normal in the vps41-deficient pollen tubes, whereas PVC-to-vacuole trafficking was impaired. We further show that the CHCR domain is required for subcellular localization and biological functioning of AtVPS41. These results indicate that the AtVPS41-mediated late stage of the endocytic pathway is essential for pollen tube-stigma interaction in Arabidopsis.; National Basic Research Program of China [2011CB915402]; National Natural Science Foundation of China [31370344]; 111 Project; SCI(E); PubMed; ARTICLE; qulj@pku.edu.cn; 22; 6307-6312; 113
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433708]
专题	生命科学学院
推荐引用方式 GB/T 7714	Hao, Lihong,Liu, Jingjing,Zhong, Sheng,et al. AtVPS41-mediated endocytic pathway is essential for pollen tube-stigma interaction in Arabidopsis[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2016.
APA	Hao, Lihong,Liu, Jingjing,Zhong, Sheng,Gu, Hongya,&Qu, Li-Jia.(2016).AtVPS41-mediated endocytic pathway is essential for pollen tube-stigma interaction in Arabidopsis.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.
MLA	Hao, Lihong,et al."AtVPS41-mediated endocytic pathway is essential for pollen tube-stigma interaction in Arabidopsis".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016).