Regulation of alkane degradation pathway by a TetR family repressor via   an autoregulation positive feedback mechanism in a Gram-positive Dietzia   bacterium

doi:10.1111/mmi.13232

CORC > 北京大学 > 生命科学学院

	Regulation of alkane degradation pathway by a TetR family repressor via an autoregulation positive feedback mechanism in a Gram-positive Dietzia bacterium
	Liang, Jie-Liang ; Nie, Yong ; Wang, Miaoxiao ; Xiong, Guangming ; Wang, Yi-Ping ; Maser, Edmund ; Wu, Xiao-Lei
刊名	MOLECULAR MICROBIOLOGY
	2016
关键词	VELOCITY ANALYTICAL ULTRACENTRIFUGATION CHAIN N-ALKANES STRUCTURAL BASIS ALCANIVORAX-BORKUMENSIS PSEUDOMONAS-OLEOVORANS HYDROXYLASE SYSTEMS SP-NOV. TRANSCRIPTIONAL REGULATION CRYSTAL-STRUCTURE GENOME SEQUENCE
DOI	10.1111/mmi.13232
英文摘要	n-Alkanes are ubiquitous in nature and serve as important carbon sources for both Gram-positive and Gram-negative bacteria. Hydroxylation of n-alkanes by alkane monooxygenases is the first and most critical step in n-alkane metabolism. However, regulation of alkane degradation genes in Gram-positive bacteria remains poorly characterized. We therefore explored the transcriptional regulation of an alkB-type alkane hydroxylase-rubredoxin fusion gene, alkW1, from Dietzia sp. DQ12-45-1b. The alkW1 promoter was characterized and so was the putative TetR family regulator, AlkX, located downstream of alkW1 gene. We further identified an unusually long 48 bp inverted repeat upstream of alkW1 and demonstrated the binding of AlkX to this operator. Analytical ultracentrifugation and microcalorimetric results indicated that AlkX formed stable dimers in solution and two dimers bound to one operator in a positive cooperative fashion characterized by a Hill coefficient of 1.64 (+/- 0.03) [k(D) = 1.06 (+/- 0.16) mu M, k(D)' = 0.05 (+/- 0.01) mu M]. However, the DNA-binding affinity was disrupted in the presence of long-chain fatty acids (C10-C24), suggesting that AlkX can sense the concentrations of n-alkane degradation metabolites. A model was therefore proposed where AlkX controls alkW1 expression in a metabolite-dependent manner. Bioinformatic analysis revealed that the alkane hydroxylase gene regulation mechanism may be common among Actinobacteria.; National Natural Science Foundation of China [31225001, 31300108]; National High Technology Research and Development Program of China [2012AA02A703]; SCI(E); PubMed; ARTICLE; xiaolei_wu@pku.edu.cn; 2; 338-359; 99
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417822]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Liang, Jie-Liang,Nie, Yong,Wang, Miaoxiao,et al. Regulation of alkane degradation pathway by a TetR family repressor via an autoregulation positive feedback mechanism in a Gram-positive Dietzia bacterium[J]. MOLECULAR MICROBIOLOGY,2016.
APA	Liang, Jie-Liang.,Nie, Yong.,Wang, Miaoxiao.,Xiong, Guangming.,Wang, Yi-Ping.,...&Wu, Xiao-Lei.(2016).Regulation of alkane degradation pathway by a TetR family repressor via an autoregulation positive feedback mechanism in a Gram-positive Dietzia bacterium.MOLECULAR MICROBIOLOGY.
MLA	Liang, Jie-Liang,et al."Regulation of alkane degradation pathway by a TetR family repressor via an autoregulation positive feedback mechanism in a Gram-positive Dietzia bacterium".MOLECULAR MICROBIOLOGY (2016).