Assembly of Customized TAL Effectors Through Advanced ULtiMATE System

CORC > 北京大学 > 生命科学学院

	Assembly of Customized TAL Effectors Through Advanced ULtiMATE System
	Yang Junjiao ; Guo Shengjie ; Yuan Pengfei ; Wei Wensheng
刊名	Methods in molecular biology (Clifton, N.J.)
	2016
关键词	Assembly,TAL effectors,TALENs,ULtiMATE system,USER
DOI	10.1007/978-1-4939-2932-0_5
英文摘要	Transcription activator-like effectors (TALEs) have been widely applied in gene targeting. Here we describe an advanced ULtiMATE (USER-based Ligation-Mediated Assembly of TAL Effector) system that utilizes USER fusion technique and archive of 512 tetramer templates to achieve highly efficient construction of TALEs, which takes only half a day to accomplish the assembly of any given TALE construct. This system is also suitable for large-scale assembly of TALENs and any other TALE-based constructions.; PubMed; 49-60; 1338
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417644]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang Junjiao,Guo Shengjie,Yuan Pengfei,et al. Assembly of Customized TAL Effectors Through Advanced ULtiMATE System[J]. Methods in molecular biology (Clifton, N.J.),2016.
APA	Yang Junjiao,Guo Shengjie,Yuan Pengfei,&Wei Wensheng.(2016).Assembly of Customized TAL Effectors Through Advanced ULtiMATE System.Methods in molecular biology (Clifton, N.J.).
MLA	Yang Junjiao,et al."Assembly of Customized TAL Effectors Through Advanced ULtiMATE System".Methods in molecular biology (Clifton, N.J.) (2016).