Differentially Expressed MicroRNA-483 Confers Distinct Functions in   Pancreatic beta- and alpha-Cells

doi:10.1074/jbc.M115.650705

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	Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells
	Mohan, Ramkumar ; Mao, Yiping ; Zhang, Shungang ; Zhang, Yu-Wei ; Xu, Cheng-Ran ; Gradwohl, Gerard ; Tang, Xiaoqing
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2015
关键词	GROWTH-FACTOR-II SWITCH-OFF HYPOTHESIS GLUCAGON-SECRETION CYTOKINE SIGNALING-3 INSULIN-RESISTANCE GLUCOSE-HOMEOSTASIS DIABETES-MELLITUS ROAD MAP PROLIFERATION APOPTOSIS
DOI	10.1074/jbc.M115.650705
英文摘要	Insulin secreted from pancreatic beta-cells and glucagon secreted from pancreatic alpha-cells are the two major hormones working in the pancreas in an opposing manner to regulate and maintain a normal glucose homeostasis. How microRNAs (miRNAs), a population of non-coding RNAs so far demonstrated to be differentially expressed in various types of cells, regulate gene expression in pancreatic beta-cells and its closely associated alpha-cells is not completely clear. In this study, miRNA profiling was performed and compared between pancreatic beta-cells and their partner alpha-cells. One novel miRNA, miR-483, was identified for its highly differential expression in pancreatic beta-cells when compared to its expression in alpha-cells. Overexpression of miR-483 in beta-cells increased insulin transcription and secretion by targeting SOCS3, a member of suppressor of cytokine signaling family. In contrast, overexpression of miR-483 decreased glucagon transcription and secretion in alpha-cells. Moreover, overexpressed miR-483 protected against proinflammatory cytokine-induced apoptosis in beta-cells. This correlates with a higher expression level of miR-483 and the expanded beta-cell mass observed in the islets of prediabetic db/db mice. Together, our data suggest that miR-483 has opposite effects in alpha- and beta-cells by targeting SOCS3, and the imbalance of miR-483 and its targets may play a crucial role in diabetes pathogenesis.; National Institutes of Health [DK084166]; SCI(E); EI; PubMed; ARTICLE; xtang2@mtu.edu; 32; 19955-19966; 290
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417254]
专题	生命科学学院
推荐引用方式 GB/T 7714	Mohan, Ramkumar,Mao, Yiping,Zhang, Shungang,et al. Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015.
APA	Mohan, Ramkumar.,Mao, Yiping.,Zhang, Shungang.,Zhang, Yu-Wei.,Xu, Cheng-Ran.,...&Tang, Xiaoqing.(2015).Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells.JOURNAL OF BIOLOGICAL CHEMISTRY.
MLA	Mohan, Ramkumar,et al."Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic beta- and alpha-Cells".JOURNAL OF BIOLOGICAL CHEMISTRY (2015).