Development of a Clickable Probe for Profiling of Protein   Glutathionylation in the Central Cellular Metabolism of E. coli and   Drosophila

doi:10.1016/j.chembiol.2015.09.012

CORC > 北京大学 > 生命科学学院

	Development of a Clickable Probe for Profiling of Protein Glutathionylation in the Central Cellular Metabolism of E. coli and Drosophila
	Feng, Shan ; Chen, Yuling ; Yang, Fan ; Zhang, Lei ; Gong, Yiyi ; Adilijiang, Gulishana ; Gao, Yan ; Deng, Haiteng
刊名	CHEMISTRY & BIOLOGY
	2015
关键词	S-GLUTATHIONYLATION GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE OXIDATIVE STRESS CREATINE-KINASE REDOX-MODIFICATIONS BINDING DOMAIN GLUTAREDOXIN IDENTIFICATION STABILITY SUBUNIT
DOI	10.1016/j.chembiol.2015.09.012
英文摘要	Protein glutathionylation is an important post-translational modification that regulates many cellular processes, including energy metabolism, signal transduction, and protein homeostasis. Global profiling of glutathionylated proteins (denoted as glutathionylome) is crucial for understanding redox-regulated signal transduction. Here, we developed a novel method based on click reaction and proteomics to enrich and identify the glutathionylated peptides in Escherichia coli and Drosophila lysates, in which 937 and 1,930 potential glutathionylated peptides were identified, respectively. Bioinformatics analysis showed that the cysteine residue next to negatively charged amino acid residues has a higher frequency of glutathionylation. Importantly, we found that most proteins associated with metabolic pathways were glutathionylated and that the glutathionylation sites of metabolic enzymes were highly conserved among different species. Our results indicate that the glutathione analog is a useful tool to characterize protein glutathionylation, and glutathionylation of metabolic enzymes, which play important roles in regulating cellular metabolism, is conserved.; NSFC [31270871, 21502103]; MOEC [2012Z02293]; China Postdoctoral Science Foundation [2015M571024]; Chinese Ministry of Science and Technology [2014CBA02005, 2014AA020907]; Global Science Alliance Program of Thermo-Fisher Scientific; SCI(E); PubMed; ARTICLE; dht@mail.tsinghua.edu.cn; 11; 1461-1469; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417085]
专题	生命科学学院
推荐引用方式 GB/T 7714	Feng, Shan,Chen, Yuling,Yang, Fan,et al. Development of a Clickable Probe for Profiling of Protein Glutathionylation in the Central Cellular Metabolism of E. coli and Drosophila[J]. CHEMISTRY & BIOLOGY,2015.
APA	Feng, Shan.,Chen, Yuling.,Yang, Fan.,Zhang, Lei.,Gong, Yiyi.,...&Deng, Haiteng.(2015).Development of a Clickable Probe for Profiling of Protein Glutathionylation in the Central Cellular Metabolism of E. coli and Drosophila.CHEMISTRY & BIOLOGY.
MLA	Feng, Shan,et al."Development of a Clickable Probe for Profiling of Protein Glutathionylation in the Central Cellular Metabolism of E. coli and Drosophila".CHEMISTRY & BIOLOGY (2015).