Usp16 regulates kinetochore localization of Plk1 to promote proper   chromosome alignment in mitosis

doi:10.1083/jcb.201502044

CORC > 北京大学 > 生命科学学院

	Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis
	Zhuo, Xiaolong ; Guo, Xiao ; Zhang, Xiaoyan ; Jing, Guihua ; Wang, Yao ; Chen, Qiang ; Jiang, Qing ; Liu, Junjun ; Zhang, Chuanmao
刊名	JOURNAL OF CELL BIOLOGY
	2015
关键词	SPINDLE ASSEMBLY CHECKPOINT CELL-CYCLE PROGRESSION POLO-LIKE KINASES MICROTUBULE ATTACHMENTS PLK1-PBIP1 INTERACTION PHOSPHORYLATION POLO-LIKE-KINASE-1 DYNAMICS PROTEIN BUBR1
DOI	10.1083/jcb.201502044
英文摘要	During the G2 to M phase transition, a portion of mitotic regulator Plk1 localizes to the kinetochores and regulates the initiation of kinetochore-microtubule attachments for proper chromosome alignment. Once kinetochore-microtubule attachment is achieved, this portion of Plk1 is removed from the kinetochores as a result of ubiquitination. However, the crucial molecular mechanism that promotes the localization and the maintenance of Plk1 on the kinetochores until metaphase is still unclear. We report that ubiquitin-specific peptidase 16 (Usp16) plays a key role during this process. Usp16 deubiquitinates Plk1, resulting in an enhanced interaction with kinetochore-localized proteins such as BubR1, and thereby retains Plk1 on the kinetochores to promote proper chromosome alignment in early mitosis. Down-regulation of Usp16 causes increased ubiquitination and decreased kinetochore localization of Plk1. Thus, our data unveil a unique mechanism by which Usp16 promotes the localization and maintenance of Plk1 on the kinetochores for proper chromosome alignment.; National Natural Science Foundation of China [91313302, 31030044, 31371365]; State Key Basic Research and Development Plan of the Ministry of Science and Technology of China [2010CB833705, 2014CB138402]; National Institutes of Health [5SC2GM089622-03]; SCI(E); PubMed; ARTICLE; junjunliu@cpp.edu; zhangcm@pku.edu.cn; 5; 727-735; 210
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417005]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhuo, Xiaolong,Guo, Xiao,Zhang, Xiaoyan,et al. Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis[J]. JOURNAL OF CELL BIOLOGY,2015.
APA	Zhuo, Xiaolong.,Guo, Xiao.,Zhang, Xiaoyan.,Jing, Guihua.,Wang, Yao.,...&Zhang, Chuanmao.(2015).Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.JOURNAL OF CELL BIOLOGY.
MLA	Zhuo, Xiaolong,et al."Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis".JOURNAL OF CELL BIOLOGY (2015).