Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin ��

doi:10.1074/jbc.M607203200

CORC > 北京大学 > 生命科学学院

	Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin ��
	Albee, Alison J. ; Tao, Wei ; Wiese, Christiane
刊名	journal of biological chemistry
	2006
DOI	10.1074/jbc.M607203200
英文摘要	Mitotic spindle assembly in Xenopus egg extracts is regulated at least in part by importin ?? and its regulator, the small GTPase, Ran. RanGTP stabilizes microtubules near the chromosomes during spindle assembly by selectively releasing spindle assembly factors from inhibition by importin ??/?? in the vicinity of the chromosomes. Several spindle assembly factors are regulated in this manner. We identified maskin, the Xenopus member of the transforming acidic coiled coil family of proteins, as a potential candidate in a two-step affinity chromatography approach designed to uncover additional downstream targets of importin ??/?? in mitosis. Here, we show that although maskin lacks a canonical nuclear localization sequence, it binds importin ?? in a RanGTP-regulated manner. We further show that importin ?? inhibits the regulatory phosphorylation of maskin by Aurora-A. This suggests a novel mechanism by which importin ?? regulates the activity of a spindle assembly factor. ? 2006 by The American Society for Biochemistry and Molecular Biology, Inc.; EI; 0; 50; 38293-38301; 281
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/410027]
专题	生命科学学院
推荐引用方式 GB/T 7714	Albee, Alison J.,Tao, Wei,Wiese, Christiane. Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin ��[J]. journal of biological chemistry,2006.
APA	Albee, Alison J.,Tao, Wei,&Wiese, Christiane.(2006).Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin ��.journal of biological chemistry.
MLA	Albee, Alison J.,et al."Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin ��".journal of biological chemistry (2006).