Cloning of a novel aldo-keto reductase gene from Klebsiella sp strain   F51-1-2 and its functional expression in Escherichia coli

doi:10.1128/AEM.02993-06

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	Cloning of a novel aldo-keto reductase gene from Klebsiella sp strain F51-1-2 and its functional expression in Escherichia coli
	Jiang, Hong ; Yang, Chao ; Qu, Hong ; Liu, Zheng ; Fu, Q. S. ; Qiao, Chuanfing
刊名	applied and environmental microbiology
	2007
关键词	PSEUDOMONAS-DIMINUTA DIHYDRODIOL DEHYDROGENASE 3-DIMENSIONAL STRUCTURE CARBONYL REDUCTION SWISS-MODEL PHOSPHOTRIESTERASE SUBSTRATE DEGRADATION PARATHION PURIFICATION
DOI	10.1128/AEM.02993-06
英文摘要	A soil bacterium capable of metabolizing organophosphorus compounds by reducing the P=S group in the molecules was taxonomically identified as Klebsiella sp. strain F51-1-2. The gene involved in the reduction of organophosphorus compounds was cloned from this strain by the shotgun technique, and the deduced protein (named AKR5FI) showed homology to members of the aldo-keto reductase (AKR) superfamily. The intact coding region for AKR5F1 was subcloned into vector pET28a and overexpressed in Escherichia coli BL21(DE3). Recombinant His(6)-tagged AKR5F1 was purified in one step using Ni-nitrilotriacetic acid affinity chromatography. Assays for cofactor specificity indicated that reductive transformation of organophosphorus compounds by the recombinant AKR5F1 specifically required NADH. The kinetic constants of the purified recombinant AKR5F1 toward six thion organophosphorus compounds were determined. For example, the K-m and k(cat) values of reductive transformation of malathion by the purified recombinant AKR5F1 are 269.5 +/- 47.0 mu M and 25.7 +/- 1.7 min(-1), respectively. Furthermore, the reductive transformation of organophosphorus compounds can be largely explained by structural modeling.; Biotechnology & Applied Microbiology; Microbiology; SCI(E); EI; 0; ARTICLE; 15; 4959-4965; 73
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397708]
专题	生命科学学院
推荐引用方式 GB/T 7714	Jiang, Hong,Yang, Chao,Qu, Hong,et al. Cloning of a novel aldo-keto reductase gene from Klebsiella sp strain F51-1-2 and its functional expression in Escherichia coli[J]. applied and environmental microbiology,2007.
APA	Jiang, Hong,Yang, Chao,Qu, Hong,Liu, Zheng,Fu, Q. S.,&Qiao, Chuanfing.(2007).Cloning of a novel aldo-keto reductase gene from Klebsiella sp strain F51-1-2 and its functional expression in Escherichia coli.applied and environmental microbiology.
MLA	Jiang, Hong,et al."Cloning of a novel aldo-keto reductase gene from Klebsiella sp strain F51-1-2 and its functional expression in Escherichia coli".applied and environmental microbiology (2007).