Reactive oxygen species involved in trichosanthin-induced apoptosis of   human choriocarcinoma cells

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	Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells
	Zhang, CY ; Gong, YX ; Ma, H ; An, CC ; Chen, DY
刊名	biochemical journal
	2001
关键词	calcium caspase-3 hydrogen peroxide hydroxyl radical superoxide radical anion HYDROGEN-PEROXIDE PRODUCTION AIDS-RELATED COMPLEX SARCOPLASMIC-RETICULUM CA2+ RELEASE ACTIVATION RADICALS CALCIUM INHIBITOR PROTEIN GLQ223
英文摘要	The type-I ribosome-inactivating protein trichosanthin (TCS) has a broad spectrum of biological and pharmacological activities, including abortifacient, anti-tumour and anti-HIV activities. We have found for the first time that TCS stimulated the production of reactive oxygen species (ROS) in JAR cells (a human choriocarcinoma cell line) in a time- and concentration-dependent manner by using the fluorescent probe 2',7'-dichlorofluorescein diacetate with confocal laser scanning microscopy. ESR spectral studies and the inhibition of ROS formation by the superoxide radical anion (O-2(-.)) scavenger superoxide dismutase, the H2O2 scavenger catalase and the hydroxyl radical (OH.) scavenger mannitol suggested the involvement of O-2(-.), H2O2 and OH.. TCS-induced ROS formation was shown to be dependent on the presence of both extracellular and intracellular Ca2+; moreover, ROS production paralleled the intracellular Ca2+ elevation induced by TCS, suggesting that ROS production might be a consequence of Ca2+ signalling. TCS-induced activation of caspase-3 was initiated within 2 h; however, TCS-induced production of ROS was initiated within 5 min, suggesting that the production of ROS preceded the activation of caspase-3. Simultaneous observation of the nuclear morphological changes via two-photon laser scanning microscopy and ROS production via confocal laser scanning microscopy revealed that ROS is involved in the apoptosis of JAR cells. The involvement of ROS was also confirmed by the inhibition of TCS-induced cell death by the antioxidant Trolox and the ROS scavengers catalase and mannitol. Diethylene-triaminepenta-acetic acid, an inhibitor of metal-facilitated OH. formation, markedly inhibited TCS-induced cell death, suggesting that TCS induced OH. formation via the Fenton reaction. The finding that ROS is involved in the TCS-induced apoptosis of JAR cells might provide new insight into the antitumour and anti-HIV mechanism of TCS.; Biochemistry & Molecular Biology; SCI(E); EI; 53; ARTICLE; 3; 653-661; 355
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397002]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, CY,Gong, YX,Ma, H,et al. Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells[J]. biochemical journal,2001.
APA	Zhang, CY,Gong, YX,Ma, H,An, CC,&Chen, DY.(2001).Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells.biochemical journal.
MLA	Zhang, CY,et al."Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells".biochemical journal (2001).