Purification and amino-terminal sequencing of a 68 kD glycopolypeptide   of the plasma membrane from maize sperm cells

CORC > 北京大学 > 生命科学学院

	Purification and amino-terminal sequencing of a 68 kD glycopolypeptide of the plasma membrane from maize sperm cells
	Xu, HP ; Cheng, J ; Yang, SJ ; Cao, ZX ; Zhang, SZ
刊名	植物学报
	1999
关键词	specific glycopeptide amino-terminal sequence Zea mays sperm cell plasma membrane IN-VITRO FERTILIZATION GLYCOPROTEINS PROTEINS ADHESION
英文摘要	Based on author's previous work on detection and immunolocalization of glycoproteins of the plasma membrane of maize (Zea mays I,.) sperm cells, a 68 kD peripheral specific glycopolypeptide of the plasma membrane from maize sperm cells was purified by IEF-SDS two-dimensional electrophoresis. It presents specifically positive reaction in Con A-HRP (concanavalin A-horseradish peroxidase) staining, and its pi value is 5.5. The search in protein sequence database reveals that the amino-terminal sequence of this glycopolypeptide is identical with that of Con A. But its difference from Con A in molecular weight and pi value indicates that it could be related to a Con A receptor on the plasma membranes of maize sperm cells instead of being Con A itself. It is fascinating to study further the function of the above glycopolypeptide in gametic recognition, adhesion and fusion of the double fertilization in maize.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000082558200011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 1; 5; 498-501; 41
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/389998]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, HP,Cheng, J,Yang, SJ,et al. Purification and amino-terminal sequencing of a 68 kD glycopolypeptide of the plasma membrane from maize sperm cells[J]. 植物学报,1999.
APA	Xu, HP,Cheng, J,Yang, SJ,Cao, ZX,&Zhang, SZ.(1999).Purification and amino-terminal sequencing of a 68 kD glycopolypeptide of the plasma membrane from maize sperm cells.植物学报.
MLA	Xu, HP,et al."Purification and amino-terminal sequencing of a 68 kD glycopolypeptide of the plasma membrane from maize sperm cells".植物学报 (1999).