Isolation of transcription factors binding auxin response elements using   a yeast one-hybrid system

doi:10.1360/02yc9020

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	Isolation of transcription factors binding auxin response elements using a yeast one-hybrid system
	Qi, M ; Huang, MJ ; Chen, F
刊名	science in china series c life sciences
	2002
关键词	auxin response elements transcription factors yeast one-hybrid system EXPRESSION CULTURE CLONING EMBRYO L.
DOI	10.1360/02yc9020
英文摘要	Plant hormones play an important role during higher plant embryogenesis. Auxin is central to the development of vascular tissues, formation of lateral and adventitious roots, control of apical dominance, and tropic responses. Auxin response element (AuxRE), present in the promoters of many auxin-induced genes, can confer auxin responsiveness. Using carrot somatic embryo under specific developmental phase, a cDNA expression library was constructed. Several plasmids were recombined containing the tetramer of AuxRE as a bait. After screening by a yeast one-hybrid system, one positive clone was confirmed and characterized. Electrophoretic mobility shift assay showed that AxRF1 protein expressed in yeast cell could bind AuxRE in vitro. It suggests that AxRF1 participates in regulation of the expression of auxin responsive gene during carrot somatic embryogenesis.; Biology; SCI(E); EI; 3; ARTICLE; 2; 177-185; 45
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/388410]
专题	生命科学学院
推荐引用方式 GB/T 7714	Qi, M,Huang, MJ,Chen, F. Isolation of transcription factors binding auxin response elements using a yeast one-hybrid system[J]. science in china series c life sciences,2002.
APA	Qi, M,Huang, MJ,&Chen, F.(2002).Isolation of transcription factors binding auxin response elements using a yeast one-hybrid system.science in china series c life sciences.
MLA	Qi, M,et al."Isolation of transcription factors binding auxin response elements using a yeast one-hybrid system".science in china series c life sciences (2002).