Dopamine release from transplanted neural stem cells in Parkinsonian rat   striatum in vivo

doi:10.1073/pnas.1408484111

CORC > 北京大学 > 生命科学学院

	Dopamine release from transplanted neural stem cells in Parkinsonian rat striatum in vivo
	Kang, Xinjiang ; Xu, Huadong ; Teng, Sasa ; Zhang, Xiaoyu ; Deng, Zijun ; Zhou, Li ; Zuo, Panli ; Liu, Bing ; Liu, Bin ; Wu, Qihui ; Wang, Li ; Hu, Meiqin ; Dou, Haiqiang ; Liu, Wei ; Zhu, Feipeng ; Li, Qing ; Guo, Shu ; Gu, Jingli ; Lei, Qian ; Lu, Jing ; Mu, Yu ; Jin, Mu ; Wang, Shirong ; Jiang, Wei ; Liu, Kun ; Wang, Changhe ; Li, Wenlin ; Zhang, Kang ; Zhou, Zhuan
刊名	proceedings of the national academy of sciences of the united states of america
	2014
关键词	dopamine Parkinson&apos striatum in vivo neural stem cells CFE CHROMAFFIN CELLS NIGRAL TRANSPLANTS PREFRONTAL CORTEX LOCUS-COERULEUS ES CELLS NEURONS DISEASE MIDBRAIN MODEL MODULATION s disease
DOI	10.1073/pnas.1408484111
英文摘要	Embryonic stem cell-based therapies exhibit great potential for the treatment of Parkinson's disease (PD) because they can significantly rescue PD-like behaviors. However, whether the transplanted cells themselves release dopamine in vivo remains elusive. We and others have recently induced human embryonic stem cells into primitive neural stem cells (pNSCs) that are self-renewable for massive/transplantable production and can efficiently differentiate into dopamine-like neurons (pNSC-DAn) in culture. Here, we showed that after the striatal transplantation of pNSC-DAn, (i) pNSC-DAn retained tyrosine hydroxylase expression and reduced PD-like asymmetric rotation; (ii) depolarization-evoked dopamine release and reuptake were significantly rescued in the striatum both in vitro (brain slices) and in vivo, as determined jointly by microdialysis-based HPLC and electrochemical carbon fiber electrodes; and (iii) the rescued dopamine was released directly from the grafted pNSC-DAn (and not from injured original cells). Thus, pNSC-DAn grafts release and reuptake dopamine in the striatum in vivo and alleviate PD symptoms in rats, providing proof-of-concept for human clinical translation.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000344088100051&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 8; ARTICLE; liwenlin@smmu.edu.cn; k5zhang@ad.ustc.edu; zzhou@pku.edu.cn; 44; 15804-15809; 111
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/387981]
专题	生命科学学院
推荐引用方式 GB/T 7714	Kang, Xinjiang,Xu, Huadong,Teng, Sasa,et al. Dopamine release from transplanted neural stem cells in Parkinsonian rat striatum in vivo[J]. proceedings of the national academy of sciences of the united states of america,2014.
APA	Kang, Xinjiang.,Xu, Huadong.,Teng, Sasa.,Zhang, Xiaoyu.,Deng, Zijun.,...&Zhou, Zhuan.(2014).Dopamine release from transplanted neural stem cells in Parkinsonian rat striatum in vivo.proceedings of the national academy of sciences of the united states of america.
MLA	Kang, Xinjiang,et al."Dopamine release from transplanted neural stem cells in Parkinsonian rat striatum in vivo".proceedings of the national academy of sciences of the united states of america (2014).