Protein preparation, crystallization and preliminary X-ray analysis of   human adrenal gland protein AD-004

doi:10.1107/S0907444904010467

CORC > 北京大学 > 生命科学学院

	Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004
	Ren, H ; Liang, YH ; Li, R ; Ding, HT ; Qiu, SH ; Lu, SY ; An, JL ; Li, LF ; Luo, M ; Zheng, XF ; Su, XD
刊名	acta crystallographica section d biological crystallography
	2004
关键词	EXPRESSION SEQUENCES CLONING
DOI	10.1107/S0907444904010467
英文摘要	The adrenal gland protein AD-004 was identified in the human adrenal gland. Full-length AD-004 contains 172 amino acids, with a predicted molecular weight of about 20 kDa. In attempts to crystallize human AD-004, the gene was subcloned into a modified pET vector, pET21-DEST, with an N-terminal His(5) tag using the Gateway cloning system, followed by protein expression in Escherichia coli strain BL21( DE3). The protein was purified in two steps to near-homogeneity and was crystallized. The crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 99.56, c = 57.19 Angstrom. A complete 2.0 Angstrom data set has been collected at a rotating-anode X-ray source and structure determination is under way.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000222177600018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 10; ARTICLE; Pt 7; 1292-1294; 60
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346888]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ren, H,Liang, YH,Li, R,et al. Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004[J]. acta crystallographica section d biological crystallography,2004.
APA	Ren, H.,Liang, YH.,Li, R.,Ding, HT.,Qiu, SH.,...&Su, XD.(2004).Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.acta crystallographica section d biological crystallography.
MLA	Ren, H,et al."Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004".acta crystallographica section d biological crystallography (2004).