In situ visualization of rDNA arrangement and its relationship with   subnucleolar structural regions in Allium sativum cell nucleolus

doi:10.1242/jcs.00323

CORC > 北京大学 > 生命科学学院

	In situ visualization of rDNA arrangement and its relationship with subnucleolar structural regions in Allium sativum cell nucleolus
	Tao, W ; Huang, BQ ; Liu, CX ; Zhai, ZH
刊名	journal of cell science
	2003
关键词	nucleolar ultrastructure DNA specific staining Ag-NOR protein rDNA transcription immunocytochemistry plant nucleolus DENSE FIBRILLAR COMPONENT RNA-POLYMERASE-I CHRISTMAS TREES RIBOSOMAL GENES IMMUNOELECTRON MICROSCOPY INSITU HYBRIDIZATION ORGANIZER REGIONS PLANT-CELLS DNA TRANSCRIPTION
DOI	10.1242/jcs.00323
英文摘要	We used a DNA-specific staining technique to show the two states of DNA component distributed in the nucleolar region of Allium sativum cells. One state is the extended DNA fiber, and the other is the condensed DNA clump. In situ hybridization demonstrated that the extended DNA fiber was an rRNA gene. Anti-fibrillarin antibody immunolabeling revealed that these rRNA genes were located in the dense fibrillar component near the fibrillar center, including at the periphery of the fibrillar center. None was in the dense fibrillar component far away from the fibrillar center. The condensed DNA clump was located in the fibrillar center. Further observations showed that the rRNA genes in the nucleolus were all arranged around the fibrillar center and associated with the DNA clumps in the fibrillar center. Results of statistical analysis showed that the distribution region of rRNA genes occupied about one-third of the total dense fibrillar component region. Ag-NOR protein showed a similar distribution pattern to that of rDNA. Immunolabeling of an anti-RNA/DNA hybrid antibody demonstrated that the transcription sites of rRNA were located at the periphery of the fibrillar center and in the dense fibrillar component near the fibrillar center, and these sites were consistent with the location and arrangement of rDNA shown in situ. These results demonstrated that transcription of rRNA takes place around the fibrillar center and at the periphery, whereas the dense fibrillar component that was far away from fibrillar center was the non-transcription region. The DNA clumps within the fibrillar center were probably the anchoring sites for rDNA arrangement.; Cell Biology; SCI(E); PubMed; 0; ARTICLE; 6; 1117-1125; 116
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346706]
专题	生命科学学院
推荐引用方式 GB/T 7714	Tao, W,Huang, BQ,Liu, CX,et al. In situ visualization of rDNA arrangement and its relationship with subnucleolar structural regions in Allium sativum cell nucleolus[J]. journal of cell science,2003.
APA	Tao, W,Huang, BQ,Liu, CX,&Zhai, ZH.(2003).In situ visualization of rDNA arrangement and its relationship with subnucleolar structural regions in Allium sativum cell nucleolus.journal of cell science.
MLA	Tao, W,et al."In situ visualization of rDNA arrangement and its relationship with subnucleolar structural regions in Allium sativum cell nucleolus".journal of cell science (2003).