AtCDC5 regulates the G2 to M transition of the cell cycle and is   critical for the function of Arabidopsis shoot apical meristem

doi:10.1038/cr.2007.71

CORC > 北京大学 > 生命科学学院

	AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem
	Lin, Zhiqiang ; Yin, Kangquan ; Zhu, Danling ; Chen, Zhangliang ; Gu, Hongya ; Qui, Lijia
刊名	细胞研究英文版
	2007
关键词	AtCDC5 G2/M phase transition shoot apical meristems WUS STM DEPENDENT KINASE SACCHAROMYCES-CEREVISIAE HOMEOBOX GENES MITOTIC ENTRY SIZE CONTROL HUMAN CDC5 THALIANA PLANT RNA PROGRESSION
DOI	10.1038/cr.2007.71
英文摘要	As a cell cycle regulator, the Myb-related CDC5 protein was reported to be essential for the G2 phase of the cell cycle in yeast and animals, but little is known about its function in plants. Here we report the functional characterization of the CDC5 gene in Arabidopsis thaliana. Arabidopsis CDC5 (AtCDC5) is mainly expressed in tissues with high cell division activity, and is expressed throughout the entire process of embryo formation. The AtCDC5 loss-of-function mutant is embryonic lethal. In order to investigate the function of AtCDC5 in vivo, we generated AtCDC5-RNAi plants in which the expression of AtCDC5 was reduced by RNA interference. We found that the G2 to M (G2/M) phase transition was affected in the AtCDC5-RNAi plants, and that endoreduplication was increased. Additionally, the maintenance of shoot apical meristem (SAM) function was disturbed in the AtCDC5-RNAi plants, in which both the WUSCHEL (WUS)CLAVATA (CLV) and the SHOOT MERISTEMLESS (STM) pathways were impaired. In situ hybridization analysis showed that the expression of STM was greatly reduced in the shoot apical cells of the AtCDC5-RNAi plants. Moreover, cyclinB1 or Histone4 was found to be expressed in some of these cells when the transcript of STM was undetectable. These results suggest that AtCDC5 is essential for the G2/M phase transition and may regulate the function of SAM by controlling the expression of STM and WUS.; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 22; ARTICLE; 9; 815-828; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345444]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lin, Zhiqiang,Yin, Kangquan,Zhu, Danling,et al. AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem[J]. 细胞研究英文版,2007.
APA	Lin, Zhiqiang,Yin, Kangquan,Zhu, Danling,Chen, Zhangliang,Gu, Hongya,&Qui, Lijia.(2007).AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem.细胞研究英文版.
MLA	Lin, Zhiqiang,et al."AtCDC5 regulates the G2 to M transition of the cell cycle and is critical for the function of Arabidopsis shoot apical meristem".细胞研究英文版 (2007).