Efficient LEC2 activation of OLEOSIN expression requires two neighboring   RY elements on its promoter

doi:10.1007/s11427-009-0119-z

CORC > 北京大学 > 生命科学学院

	Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter
	Che NanYing ; Yang Yang ; Li YanDong ; Wang LiLi ; Huang Ping ; Gao Yin ; An ChengCai
刊名	science in china series c life sciences
	2009
关键词	OLEOSIN LEC2 gene expression seed-specific gene RY element B3 domain transcription factor BRASSICA-NAPUS ARABIDOPSIS-THALIANA GENE-EXPRESSION ABSCISIC-ACID OIL BODIES PROTEIN SEEDS MATURATION REPEATS
DOI	10.1007/s11427-009-0119-z
英文摘要	As the main structural protein of oil body, OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here, we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN, responsible for seed specificity and LEC2 activation, was determined by 5'-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays, mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity, while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore, we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000270489500010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biology; SCI(E); PubMed; 8; ARTICLE; 9; 854-863; 52
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/344801]
专题	生命科学学院
推荐引用方式 GB/T 7714	Che NanYing,Yang Yang,Li YanDong,et al. Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter[J]. science in china series c life sciences,2009.
APA	Che NanYing.,Yang Yang.,Li YanDong.,Wang LiLi.,Huang Ping.,...&An ChengCai.(2009).Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter.science in china series c life sciences.
MLA	Che NanYing,et al."Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter".science in china series c life sciences (2009).