Generation of Homogeneous PDX1(+) Pancreatic Progenitors from Human ES   Cell-derived Endoderm Cells

doi:10.1093/jmcb/mjp037

CORC > 北京大学 > 生命科学学院

	Generation of Homogeneous PDX1(+) Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells
	Cai, Jun ; Yu, Chen ; Liu, Yanxia ; Chen, Song ; Guo, Yuxuan ; Yong, Jun ; Lu, Wei ; Ding, Mingxiao ; Deng, Hongkui
刊名	分子细胞生物学报
	2010
关键词	embryonic stem cell retinoic acid PDX1 pancreatic progenitor hepatic Smad EMBRYONIC STEM-CELLS BONE MORPHOGENETIC PROTEINS RETINOIC ACID DEFINITIVE ENDODERM BETA-CELLS EFFICIENT DIFFERENTIATION DIRECTED DIFFERENTIATION ISLET TRANSPLANTATION MOUSE ENDOCRINE
DOI	10.1093/jmcb/mjp037
英文摘要	One key step in producing insulin-secreting cells from human embryonic stem (hES) cells is the generation of pancreatic and duodenal homeobox gene 1 (PDX1)-expressing pancreatic progenitor cells. All-trans retinoic acid (RA) has important roles in pancreas development and is widely used to induce pancreatic differentiation of ES cells. When RA was added directly to the activin A-induced hES cells, <20% cells were positive for the pancreatic marker PDX1, whereas the other cells were mainly hepatic cells. We found that when the activin A-induced hES cells were replated and seeded at low cell densities, the addition of RA induced significant pancreatic differentiation and over 70% of cells in culture expressed PDX1. When the endodermal cells were isolated with the surface marker CXCR4 from the activin A-induced culture and further differentiated with RA, a homogeneous PDX1(+) cell population (over 95% pure) was generated. The PDX1(+) cells could further differentiate into cells that expressed pancreatic transcription factors and pancreatic endocrine or exocrine markers. We also found that RA inhibited the hepatic differentiation of endodermal cells that were seeded at low cell densities, and this inhibition may have been through the inhibition of Smad1/5/8 activity. Thus, we present a highly efficient and reproducible protocol for generating PDX1(+) pancreatic progenitor cells from hES cells.; Cell Biology; SCI(E); PubMed; 中国科学引文数据库(CSCD); 0; ARTICLE; 1; 50-60; 2
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/344729]
专题	生命科学学院
推荐引用方式 GB/T 7714	Cai, Jun,Yu, Chen,Liu, Yanxia,et al. Generation of Homogeneous PDX1(+) Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells[J]. 分子细胞生物学报,2010.
APA	Cai, Jun.,Yu, Chen.,Liu, Yanxia.,Chen, Song.,Guo, Yuxuan.,...&Deng, Hongkui.(2010).Generation of Homogeneous PDX1(+) Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells.分子细胞生物学报.
MLA	Cai, Jun,et al."Generation of Homogeneous PDX1(+) Pancreatic Progenitors from Human ES Cell-derived Endoderm Cells".分子细胞生物学报 (2010).