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	Mutational analysis of dimeric linkers in peri- and cytoplasmic domains of histidine kinase DctB reveals their functional roles in signal transduction
	Liu, Jiwei ; Yang, Jianguo ; Wen, Jin ; Yang, Yun ; Wei, Xiaolu ; Zhang, Xiaodong ; Wang, Yi-Ping
	2014
关键词	DctB two-component system transmembrane signal transduction MULTIPLE SEQUENCE ALIGNMENT COILED COILS GENETIC-ANALYSIS SENSOR KINASES PROTEINS MECHANISM HELIX TRANSPORT CHEMORECEPTORS TRANSMISSION
DOI	10.1098/rsob.140023
英文摘要	Membrane-associated histidine kinases (HKs) in two-component systems respond to environmental stimuli by autophosphorylation and phospho-transfer. HK typically contains a periplasmic sensor domain that regulates the cytoplasmic kinase domain through a conserved cytoplasmic linker. How signal is transduced from the ligand-binding site across the membrane barrier remains unclear. Here, we analyse two linker regions of a typical HK, DctB. One region connects the first transmembrane helix with the periplasmic Per-ARNT-Sim domains, while the other one connects the second transmembrane helix with the cytoplasmic kinase domains. We identify a leucine residue in the first linker region to be essential for the signal transduction and for maintaining the delicate balance of the dimeric interface, which is key to its activities. We also show that the other linker, belonging to the S-helix coiled-coil family, plays essential roles in signal transduction inside the cell. Furthermore, by combining mutations with opposing activities in the two regions, we show that these two signalling transduction elements are integrated to produce a combined effect on the final activity of DctB.; Biochemistry & Molecular Biology; SCI(E); PubMed; 0; ARTICLE; xiaodong.zhang@imperial.ac.uk; wangyp@pku.edu.cn; 6; 140023; 4
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342151]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Jiwei,Yang, Jianguo,Wen, Jin,et al. Mutational analysis of dimeric linkers in peri- and cytoplasmic domains of histidine kinase DctB reveals their functional roles in signal transduction[J],2014.
APA	Liu, Jiwei.,Yang, Jianguo.,Wen, Jin.,Yang, Yun.,Wei, Xiaolu.,...&Wang, Yi-Ping.(2014).Mutational analysis of dimeric linkers in peri- and cytoplasmic domains of histidine kinase DctB reveals their functional roles in signal transduction..
MLA	Liu, Jiwei,et al."Mutational analysis of dimeric linkers in peri- and cytoplasmic domains of histidine kinase DctB reveals their functional roles in signal transduction".(2014).

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