The proteasomal inhibitor MG132 increases the efficiency of mouse embryo   production after cloning by electrofusion

doi:10.1530/rep.1.00758

CORC > 北京大学 > 生命科学学院

	The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion
	Yu, YS ; Yong, J ; Li, XY ; Qing, TT ; Qin, H ; Xiong, XR ; You, JF ; Ding, MX ; Deng, HK
刊名	reproduction
	2005
关键词	FULL-TERM DEVELOPMENT FREE CULTURE-MEDIUM NUCLEAR TRANSFER IN-VITRO PREIMPLANTATION EMBRYOS ENUCLEATED OOCYTES GENE-EXPRESSION 2-CELL BLOCK AMINO-ACIDS CELL-NUCLEI
DOI	10.1530/rep.1.00758
英文摘要	In this study, we cloned mice from ES cells by a post-electrofusion MG132 treatment and improved development of cloned embryos with a sequential cultivation protocol. When 5 mu M MG132, a proteasome inhibitor, were used to treat the reconstructed embryos, the capacity of in vitro development, implantation and full-term development were significantly improved. Blastocyst formation rates of the reconstructed embryos from X4 ES cells (F1 strain derived from C57BL/6 X 129sv) and J1 ES cells obtained with or without MG132 treatment were 66.9% and 26.6%, and 66.1% and 34.5% respectively (P < 0.05). A total of 146 two-cell embryos cloned from X4 ES cells with MG132 treatment were transferred to recipients, and five cloned pups (3.4%) were born, of which four survived. When the same numbers of two-cell embryos cloned from X4 ES cells without MG132 treatment were transferred, however, no live-born mice were obtained. When embryos cloned from J1 ES cells without MG132 treatment were cultured in KSOM medium for 54 h followed by culture in CZB medium containing 5.6 mM glucose for 42 h, the blastocyst rate was significantly higher than when they were cultured in KSOM continuously for 96 h (34.5% vs 17.1%). However, sequential cultivation did not improve the development of embryos cloned with MG132 treatment and that of parthenotes. In conclusion, MG132 treatment increased the developmental potential of reconstructed mouse embryos, and sequential cultivation improved development of the embryos cloned by electrofusion without MG132 treatment.; Developmental Biology; Reproductive Biology; SCI(E); PubMed; 9; ARTICLE; 4; 553-558; 130
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/340997]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yu, YS,Yong, J,Li, XY,et al. The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion[J]. reproduction,2005.
APA	Yu, YS.,Yong, J.,Li, XY.,Qing, TT.,Qin, H.,...&Deng, HK.(2005).The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion.reproduction.
MLA	Yu, YS,et al."The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion".reproduction (2005).