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	ALPHA-DOMAIN OF HUMAN METALLOTHIONEIN IA CAN BIND TO METALS IN TRANSGENIC TOBACCO PLANTS
	PAN, AH ; TIE, F ; DUAU, ZW ; YANG, MZ ; WANG, ZX ; LI, LY ; CHEN, ZL ; RU, BG
	1994
关键词	HUMAN METALLOTHIONEIN GENE ALPHA-DOMAIN-TRANSGENIC PLANTS HEAVY METAL TOLERANCE GENES TRANSFORMATION CADMIUM
英文摘要	With a view to exploring its use as a metal-binding factor in transgenic plants we prepared the alpha-domain of metallothionein by reconstitution of rabbit apometallothionein and proteolysis of MT-1 and MT-2 with subtilisin. The isolated alpha-domains were characterised by UV and CD spectroscopy Double-Stranded. DNA encoding the alpha-domain (106 bp) of the human MT-I(A) was constructed from chemically synthesized oligomers by repair synthesis and enzymatic ligation, cloned into pUC19 and sequenced. A expression construct containing the cloned alpha-domain was introduced into tobacco cells on a disarmed Agrobacterium tumefaciens Ti-plasmid. Transformed tobacco cells were selected and regenerated on medium containing cadmium and kanamycin. The growth of roots and shoots of transformants was unaffected by up to 100 muM cadmium, whereas control plants showed severe inhibition of root and shoot growth, and chlorosis of leaves on medium containing only 10 muM cadmium. Southern hybridization confirmed the presence of the transgene in the transformed plant tissues. The concentration of human alpha-domain peptides in transgenic tobacco eaves was determined by the Cd/hemoglobin saturation assay and polarography using the rabbit alpha-domain as standard. The results indicate that the alpha-domain, one of two domains in MT molecules, is not only stable in vitro, but is also expressed efficiently and functions independently in transgenic plant cells.; Biochemistry & Molecular Biology; Genetics & Heredity; SCI(E); 25; 6; 666-674; 242
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320325]
专题	生命科学学院
推荐引用方式 GB/T 7714	PAN, AH,TIE, F,DUAU, ZW,et al. ALPHA-DOMAIN OF HUMAN METALLOTHIONEIN IA CAN BIND TO METALS IN TRANSGENIC TOBACCO PLANTS[J],1994.
APA	PAN, AH.,TIE, F.,DUAU, ZW.,YANG, MZ.,WANG, ZX.,...&RU, BG.(1994).ALPHA-DOMAIN OF HUMAN METALLOTHIONEIN IA CAN BIND TO METALS IN TRANSGENIC TOBACCO PLANTS..
MLA	PAN, AH,et al."ALPHA-DOMAIN OF HUMAN METALLOTHIONEIN IA CAN BIND TO METALS IN TRANSGENIC TOBACCO PLANTS".(1994).

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