CLONING AND SEQUENCING OF TRICHOSANTHIN GENE AND ITS EXPRESSION IN   ESCHERICHIA-COLI AND TOBACCO PLANT

CORC > 北京大学 > 生命科学学院

	CLONING AND SEQUENCING OF TRICHOSANTHIN GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND TOBACCO PLANT
	BAO, YM ; CHU, RY ; HAN, JH ; ZHANG, H ; PAN, NS ; GU, XC ; CHEN, ZL
刊名	中国科学b辑化学英文版
	1993
关键词	TRICHOSANTHIN POLYMERASE CHAIN REACTION NUCLEOTIDE SEQUENCE ANALYSIS GENE EXPRESSION TRANSGENIC PLANT RICIN-A-CHAIN ALPHA-TRICHOSANTHIN PROTEIN KIRILOWII HOMOLOGY
英文摘要	A Trichosanthin gene was cloned from Trichosanthes kirilowii genomic DNA by polymerase chain reaction (PCR). Nucleotide sequence data indicated that we obtained the coding region of the mature Trichosanthin peptide as well as its signal peptide at the N-terminus. Comparisons of our sequence with the previously reported nucleotide sequences of this gene showed 99.25% homology, yet there were notable differences between the previously reported amino acid sequence and our deduced result. This gene was subcloned into a high-level expression plasmid (pJLA502) of E. coli under the control of a P(R)P(L) promoter, 2nd we observed the gene product after temperature induction. The gene was further cloned into plant intermediate vector pE3 under the control of a CaMV 35S promoter, and transferred into a tobacco genome using the agrobacterium-mediated gene transfer system. Western blotting analysis of the protein extracted from Escherichia coli and transgenic tobacco plants proved that the Trichosanthin gene has been expressed in both organisms.; Chemistry, Multidisciplinary; SCI(E); 4; ARTICLE; 6; 669-676; 36
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320313]
专题	生命科学学院
推荐引用方式 GB/T 7714	BAO, YM,CHU, RY,HAN, JH,et al. CLONING AND SEQUENCING OF TRICHOSANTHIN GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND TOBACCO PLANT[J]. 中国科学b辑化学英文版,1993.
APA	BAO, YM.,CHU, RY.,HAN, JH.,ZHANG, H.,PAN, NS.,...&CHEN, ZL.(1993).CLONING AND SEQUENCING OF TRICHOSANTHIN GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND TOBACCO PLANT.中国科学b辑化学英文版.
MLA	BAO, YM,et al."CLONING AND SEQUENCING OF TRICHOSANTHIN GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND TOBACCO PLANT".中国科学b辑化学英文版 (1993).