Cyclin B1 is localized to unattached kinetochores and contributes to   efficient microtubule attachment and proper chromosome alignment during   mitosis

doi:10.1038/cr.2008.11

CORC > 北京大学 > 生命科学学院

	Cyclin B1 is localized to unattached kinetochores and contributes to efficient microtubule attachment and proper chromosome alignment during mitosis
	Chen, Qiang ; Zhang, Xiaoyan ; Jiang, Qing ; Clarke, Paul R. ; Zhang, Chuamnao
刊名	细胞研究英文版
	2008
关键词	cyclin B1 kinetochore dynein chromosome alignment microtubule attachment SPINDLE-CHECKPOINT STARFISH OOCYTES SOMATIC-CELLS COMPLEX PHOSPHORYLATION DYNAMICS PROTEIN KINASE B1 ASSOCIATION
DOI	10.1038/cr.2008.11
英文摘要	Cyclin B1 is a key regulatory protein controlling cell cycle progression in vertebrates. Cyclin B1 binds,CDK1, a cyclin-dependent kinase catalytic subunit, forming a complex that orchestrates mitosis through phosphorylation of key proteins. Cyclin B1 regulates both the activation of CDK1 and its subcellular localization, which may be critical for substrate selection. Here, we demonstrate that cyclin B1 is concentrated on the outer plate of the kinetochore during prometaphase. This localization requires the cyclin box region of the protein. Cyclin B1 is displaced from individual kinetochores to the spindle poles by microtubule attachment to the kinetochores, and this displacement is dependent on the dynein/dynactin complex. Depletion of cyclin B1 by vector-based siRNA causes inefficient attachment between kinetochores and microtubules, and chromosome alignment defects, and delays the onset of anaphase. We conclude that cyclin B1 accumulates at kinetochores during prometaphase, where it contributes to the correct attachment of microtubules to kinetochores and efficient alignment of the chromosomes, most likely through localized phosphorylation of specific substrates by cyclin B1-CDK1. Cyclin B1 is then transported from each kinetochore as microtubule attachment is completed, and this relocalization may redirect the activity of cyclin B1-CDK1 and contribute to inactivation of the spindle assembly checkpoint.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000254024700008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 28; ARTICLE; 2; 268-280; 18
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/320029]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, Qiang,Zhang, Xiaoyan,Jiang, Qing,et al. Cyclin B1 is localized to unattached kinetochores and contributes to efficient microtubule attachment and proper chromosome alignment during mitosis[J]. 细胞研究英文版,2008.
APA	Chen, Qiang,Zhang, Xiaoyan,Jiang, Qing,Clarke, Paul R.,&Zhang, Chuamnao.(2008).Cyclin B1 is localized to unattached kinetochores and contributes to efficient microtubule attachment and proper chromosome alignment during mitosis.细胞研究英文版.
MLA	Chen, Qiang,et al."Cyclin B1 is localized to unattached kinetochores and contributes to efficient microtubule attachment and proper chromosome alignment during mitosis".细胞研究英文版 (2008).