Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins   for nuclear envelope assembly via importin-alpha/beta

doi:10.1038/cr.2012.113

CORC > 北京大学 > 生命科学学院

	Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins for nuclear envelope assembly via importin-alpha/beta
	Lu, Quanlong ; Lu, Zhigang ; Liu, Qinying ; Guo, Li ; Ren, He ; Fu, Jingyan ; Jiang, Qing ; Clarke, Paul R. ; Zhang, Chuanmao
刊名	细胞研究英文版
	2012
关键词	nuclear assembly nucleoplasmin Ran GTPase nuclear pore complex importin XENOPUS EGG EXTRACTS LAMIN-B RECEPTOR RAN GTPASE SPERM CHROMATIN CELL-CYCLE COMPLEX NUCLEOPLASMIN BETA DECONDENSATION FUSION
DOI	10.1038/cr.2012.113
英文摘要	The mechanism for nuclear envelope (NE) assembly is not fully understood. Importin-beta and the small GTPase Ran have been implicated in the spatial regulation of NE assembly process. Here we report that chromatin-bound NLS (nuclear localization sequence) proteins provide docking sites for the NE precursor membrane vesicles and nucleoporins via importin-alpha and -beta during NE assembly in Xenopus egg extracts. We show that along with the fast recruitment of the abundant NLS proteins such as nucleoplasmin and histones to the demembranated sperm chromatin in the extracts, importin-alpha binds the chromatin NLS proteins rapidly. Meanwhile, importin-beta binds cytoplasmic NE precursor membrane vesicles and nucleoporins. Through interacting with importin-alpha on the chromatin NLS proteins, importin-beta targets the membrane vesicles and nucleoporins to the chromatin surface. Once encountering Ran-GTP on the chromatin generated by RCC1, importin-beta preferentially binds Ran-GTP and releases the membrane vesicles and nucleoporins for NE assembly. NE assembly is disrupted by blocking the interaction between importin-alpha and NLS proteins with excess soluble NLS proteins or by depletion of importin-beta from the extract. Our findings reveal a novel molecular mechanism for NE assembly in Xenopus egg extracts.; Cell Biology; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 6; ARTICLE; 11; 1562-1575; 22
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319946]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lu, Quanlong,Lu, Zhigang,Liu, Qinying,et al. Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins for nuclear envelope assembly via importin-alpha/beta[J]. 细胞研究英文版,2012.
APA	Lu, Quanlong.,Lu, Zhigang.,Liu, Qinying.,Guo, Li.,Ren, He.,...&Zhang, Chuanmao.(2012).Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins for nuclear envelope assembly via importin-alpha/beta.细胞研究英文版.
MLA	Lu, Quanlong,et al."Chromatin-bound NLS proteins recruit membrane vesicles and nucleoporins for nuclear envelope assembly via importin-alpha/beta".细胞研究英文版 (2012).