Single-cell RNA-Seq profiling of human preimplantation embryos and   embryonic stem cells

doi:10.1038/nsmb.2660

CORC > 北京大学 > 生命科学学院

	Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells
	Yan, Liying ; Yang, Mingyu ; Guo, Hongshan ; Yang, Lu ; Wu, Jun ; Li, Rong ; Liu, Ping ; Lian, Ying ; Zheng, Xiaoying ; Yan, Jie ; Huang, Jin ; Li, Ming ; Wu, Xinglong ; Wen, Lu ; Lao, Kaiqin ; Li, Ruiqiang ; Qiao, Jie ; Tang, Fuchou
刊名	自然结构与分子生物学
	2013
关键词	LONG NONCODING RNAS GENE-EXPRESSION PROFILES TRANSCRIPTOME ANALYSIS HUMAN TROPHECTODERM BLASTOCYST GENOME MOUSE PLURIPOTENCY DERIVATION ALIGNMENT
DOI	10.1038/nsmb.2660
英文摘要	Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. Here we apply single-cell RNA sequencing (RNA-Seq) analysis to 124 individual cells from human preimplantation embryos and human embryonic stem cells (hESCs) at different passages. The number of maternally expressed genes detected in our data set is 22,687, including 8,701 long noncoding RNAs (lncRNAs), which represents a significant increase from 9,735 maternal genes detected previously by cDNA microarray. We discovered 2,733 novel lncRNAs, many of which are expressed in specific developmental stages. To address the long-standing question whether gene expression signatures of human epiblast (EPI) and in vitro hESCs are the same, we found that EPI cells and primary hESC outgrowth have dramatically different transcriptomes, with 1,498 genes showing differential expression between them. This work provides a comprehensive framework of the transcriptome landscapes of human early embryos and hESCs.; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); PubMed; 86; ARTICLE; 9; 1131-+; 20
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/309296]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yan, Liying,Yang, Mingyu,Guo, Hongshan,et al. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells[J]. 自然结构与分子生物学,2013.
APA	Yan, Liying.,Yang, Mingyu.,Guo, Hongshan.,Yang, Lu.,Wu, Jun.,...&Tang, Fuchou.(2013).Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells.自然结构与分子生物学.
MLA	Yan, Liying,et al."Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells".自然结构与分子生物学 (2013).