Quantitative study of organelle nucleoids during the second pollen grain   mitosis in Oenothera biennis

doi:10.1007/BF01282132

CORC > 北京大学 > 生命科学学院

	Quantitative study of organelle nucleoids during the second pollen grain mitosis in Oenothera biennis
	Sodmergen ; Kuroiwa, H ; Nagata, N ; Qian, ZH ; Liu, H ; Guan, XL ; Kuroiwa, T
刊名	protoplasma
	1997
关键词	plastid nucleoid mitochondrial nucleoid second pollen grain mitosis Oenothera biennis biparental cytoplasmic inheritance SPERM CELLS PLUMBAGO-ZEYLANICA MITOCHONDRIAL-DNA HIGHER-PLANTS CYTOLOGY ULTRASTRUCTURE FERTILIZATION ORGANIZATION MICROSCOPY
DOI	10.1007/BF01282132
英文摘要	The behavior of organelle nucleoids in the generative cell was examined at the second (pollen grain) mitosis by epifluorescence microscopy after staining with 4',6-diamidino-2-phenylindole (DAPI) in Oenothera biennis. The O. biennis generative cell contained a large number of organelle nucleoids distributed randomly in the cytoplasm before mitosis. The epifluorescence images of the nucleoids could be classified distinctly into two groups which corresponded to plastid nucleoids (pt-nucleoids) and mitochondrial nucleoids (mt-nucleoids). Discrimination between pt- and mt-nucleoids was carried out with the aid of DNA immunogold electron microscopy. At metaphase, both pt- and mt-nucleoids migrated to the pole regions of the generative cell. After mitosis, organelle nucleoids in both of the sperm cells scattered in the cytoplasm again. A quantitative examination of pt-nucleoids on 202 pairs of sperm cells showed that the leading sperm cell (Svn) contained 0-39 pt-nucleoids (19.0 +/- 7.4) and the trailing sperm cell (Sua) contained 0-40 pt-nucleoids (15.4 +/- 6.5). For mt-nucleoids, examination of 28 pairs of sperm cells showed that Svn contained 5-32 mt-nucleoids (14.5 +/- 6.8) and Sua contained 6-30 mt-nucleoids (13.4 +/- 7.5). These results showed that (1) the number of organelle nucleoids per sperm cell varied considerably in the cells studied; (2) quantitative difference in pt- and mt-nucleoids between Syn and Sua could occur in some gametophytes studied; but (3) it was unlikely that there was any pre-differentiational cytoplasm localization and essential sperm heteromorphy with respect to organelle nucleoid content in the gametophyte population.; Plant Sciences; Cell Biology; SCI(E); 2; ARTICLE; 1-2; 66-72; 198
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/258206]
专题	生命科学学院
推荐引用方式 GB/T 7714	Sodmergen,Kuroiwa, H,Nagata, N,et al. Quantitative study of organelle nucleoids during the second pollen grain mitosis in Oenothera biennis[J]. protoplasma,1997.
APA	Sodmergen.,Kuroiwa, H.,Nagata, N.,Qian, ZH.,Liu, H.,...&Kuroiwa, T.(1997).Quantitative study of organelle nucleoids during the second pollen grain mitosis in Oenothera biennis.protoplasma.
MLA	Sodmergen,et al."Quantitative study of organelle nucleoids during the second pollen grain mitosis in Oenothera biennis".protoplasma (1997).