Urothelial hinge as a highly specialized membrane:   detergent-insolubility, urohingin association, and in vitro formation

doi:10.1046/j.1432-0436.1999.6510059.x

CORC > 北京大学 > 生命科学学院

	Urothelial hinge as a highly specialized membrane: detergent-insolubility, urohingin association, and in vitro formation
	Liang, FX ; Kachar, B ; Ding, MX ; Zhai, ZH ; Wu, XR ; Sun, TT
刊名	differentiation
	1999
关键词	bladder epithelium urothelium uroplakin differentiation quick-freeze deep-etch MAMMALIAN URINARY-BLADDER ASYMMETRIC UNIT MEMBRANE 3-DIMENSIONAL STRUCTURE PLASMA-MEMBRANE UROPLAKIN-I DIFFERENTIATION CELLS EPITHELIUM EXPRESSION PROTEINS
DOI	10.1046/j.1432-0436.1999.6510059.x
英文摘要	Urothelial surface is covered by numerous plaques (consisting of asymmetric unit membranes or AUM) that are interconnected by ordinary looking hinge membranes. We describe an improved method for purifying bovine urothelial plaques using 2% sarkosyl and 25 mM NaOH to remove contaminating membrane and peripheral proteins selectively. Highly purified plaques interconnected by intact hinge areas were obtained, indicating that the hinges are as detergent-insoluble as the plaques. These plaque/hinge preparations contained uroplakins, an as yet uncharacterized 18-kDa plaque-associated protein, plus an 85-kDa glycoprotein that is known to be hinge-associated in situ. Examination of the isolated, in vitro-resealed bovine AUM vesicles by quick freeze deep-etch showed that each AUM particle consists of a 16-nm, luminally exposed "head" anchored to the lipid bilayer via a 9-nm transmembranous "tail", and that an AUM plaque can break forming several smaller plaques separated by newly formed particle-free, hingelike areas. These data lend support to our recently proposed three-dimensional model of mouse urothelial plaques. In addition, our findings suggest that urothelial plaques are dynamic structures that can rearrange giving rise to new plaques with intervening hinges; that the entire urothelial apical surface (both plaque and hinge areas) is highly specialized; and that these two membrane domains may be equally important in fulfilling some of the urothelial functions.; Cell Biology; Developmental Biology; SCI(E); 0; ARTICLE; 1; 59-69; 65
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/257799]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liang, FX,Kachar, B,Ding, MX,et al. Urothelial hinge as a highly specialized membrane: detergent-insolubility, urohingin association, and in vitro formation[J]. differentiation,1999.
APA	Liang, FX,Kachar, B,Ding, MX,Zhai, ZH,Wu, XR,&Sun, TT.(1999).Urothelial hinge as a highly specialized membrane: detergent-insolubility, urohingin association, and in vitro formation.differentiation.
MLA	Liang, FX,et al."Urothelial hinge as a highly specialized membrane: detergent-insolubility, urohingin association, and in vitro formation".differentiation (1999).