Transgenic expression of a putative calcium transporter affects the time   of Arabidopsis flowering

doi:10.1046/j.1365-313X.2003.01627.x

CORC > 北京大学 > 生命科学学院

	Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering
	Wang, DY ; Xu, YJ ; Li, Q ; Hao, XM ; Cui, KM ; Sun, FZ ; Zhu, YX
刊名	plant journal
	2003
关键词	PPF1 calcium fluorescence gene expression Arabidopsis transformation flowering Pisum G2 CLOCK-CONTROLLED GENE PROTEIN PLANTS ENCODES VERNALIZATION INTEGRATION GIBBERELLIN ACTIVATION TRANSITION MEMBRANES
DOI	10.1046/j.1365-313X.2003.01627.x
英文摘要	PPF1 is a gibberellin-induced, vegetative growth-specific gene, first isolated from short-day (SD)-grown G2 pea plants. In the current work, we found that transgenic Arabidopsis plants overexpressing the PPF1 gene (PPF1 (+)) flowered much later and had a significantly longer lifespan compared to control plants, whereas suppression of this gene (PPF1 (-)) resulted in a very rapid reproductive cycle. Western blotting analyses of PPF1 (+) and (-) plant lines revealed a positive correlation between the amount of antibody-reactive protein and the time of flowering. Green flourescent protein (GFP) co-expression assays showed that the PPF1 protein is likely localized in chloroplast membranes. Transgenic expression of PPF1 affected the calcium storage capacities since chloroplasts isolated from PPF1 (+) plants contained high Ca2+ levels while chloroplasts of PPF1 (-) plants contained very low amounts of calcium ion. Using Novikoff human hepatoma cells, we demonstrated that expression of PPF1 leads to a significant inward calcium ion current that was absent in untransformed cells. We conclude that, as a putative calcium ion carrier, PPF1 affects the flowering time of higher plants by modulating Ca2+ storage capacity within chloroplasts.; Plant Sciences; SCI(E); EI; 20; 2; 285-292; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256338]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, DY,Xu, YJ,Li, Q,et al. Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering[J]. plant journal,2003.
APA	Wang, DY.,Xu, YJ.,Li, Q.,Hao, XM.,Cui, KM.,...&Zhu, YX.(2003).Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering.plant journal.
MLA	Wang, DY,et al."Transgenic expression of a putative calcium transporter affects the time of Arabidopsis flowering".plant journal (2003).