The induction of Sinorhizobium meliloti C-4-dicarboxylate transport   system (Dct) is regulated by oxygen concentration

doi:10.1369/982004-174

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	The induction of Sinorhizobium meliloti C-4-dicarboxylate transport system (Dct) is regulated by oxygen concentration
	Wen, J ; Nan, BY ; O&apos ; Wang, YP ; Gara, F
刊名	科学通报英文版
	2005
关键词	Sinorhizobium meliloti Dct system oxygen concentration RHIZOBIUM-MELILOTI TRANSCRIPTIONAL ACTIVATION COOPERATIVE BINDING NITROGEN-FIXATION GENETIC-ANALYSIS ACID TRANSPORT EXPRESSION MUTANTS PROTEIN LEGUMINOSARUM
DOI	10.1369/982004-174
英文摘要	The Sinorhizobium meliloti C-4-dicarboxylate transport (Dct) system is essential for symbiotic nitrogen fixation. The dctA gene, encoding the C4-dicarboxylate permease, is expressed in both free living and symbiotic cells. But in free living cells expression of dctD and dctB is absolutely required for the expression of dctA. In this study, in order to investigate the effect of oxygen concentration on the induction of Dct system, E. coli DH5 alpha strain which carries the plasmid-encoded dctABD operon was used in tube assays. It was found that the specific induction of Dct system occurred only at a certain depth under the surface of M63-0.6% agar media, suggesting that Dct system could respond to oxygen concentration during succinate-induced expression. Furthermore, when measured at different oxygen concentrations, the highest expression level was observed at oxygen concentration of 2%. Thus, we predict that in addition to dicarboxylates, the induction of Dct system may also regulated by oxygen concentration.; Multidisciplinary Sciences; SCI(E); 0; ARTICLE; 17; 1891-1895; 50
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/253066]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wen, J,Nan, BY,O&apos,et al. The induction of Sinorhizobium meliloti C-4-dicarboxylate transport system (Dct) is regulated by oxygen concentration[J]. 科学通报英文版,2005.
APA	Wen, J,Nan, BY,O&apos,Wang, YP,&Gara, F.(2005).The induction of Sinorhizobium meliloti C-4-dicarboxylate transport system (Dct) is regulated by oxygen concentration.科学通报英文版.
MLA	Wen, J,et al."The induction of Sinorhizobium meliloti C-4-dicarboxylate transport system (Dct) is regulated by oxygen concentration".科学通报英文版 (2005).