Characterization of three Arabidopsis AP2/EREBP family transcription   factors involved in ABA sensitivity, freeze and salt tolerance

doi:10.1007/s11434-007-0276-z

CORC > 北京大学 > 生命科学学院

	Characterization of three Arabidopsis AP2/EREBP family transcription factors involved in ABA sensitivity, freeze and salt tolerance
	Mei WenQian ; Lei Juan ; Xu Yu ; Wei Gang ; Zhu YuXian
刊名	科学通报英文版
	2007
关键词	AP2/EREBP transcription factor ABA freeze tolerance salt tolerance DNA-BINDING DOMAIN ABSCISIC-ACID LOW-TEMPERATURE ETHYLENE RESPONSE STRESS TOLERANCE GENE-EXPRESSION OSMOTIC-STRESS LEAFY PETIOLE OVEREXPRESSION ACTIVATION
DOI	10.1007/s11434-007-0276-z
英文摘要	AP2/EREBP transcription factors (TFs) play very important roles in plant development, hormonal regulation and stress response. Upon genome-wide cDNA cloning, phylogenetic and expression pattern analyses of this plant specific TF family, we found that three of the members including At1g71450, At1g50680 and At5g13910, were likely involved in responses to ABA, cold and salt. Complementary DNAs containing putative full-length ORFs of these three TFs were obtained and fused individually to the GAL4 DNA-binding domains. All the 3 genes functioned effectively as trans-activators using yeast one-hybrid assays. RT-PCR experiments showed that the At1g71450 gene was induced by ABA and low temperature; the At1g50680 gene was responsive to quite a few stress conditions, but especially to freezing temperature; and the At5g13910 gene was induced by high salt treatment, drought and ethylene: By searching the ABRC T-DNA insertion mutant stocks, we obtained knockout lines for these TFs. Homozygous ko1 (At1g71450) plants showed a hypersensitive response to ABA during seed germination and also in stomata movement. Homozygous ko2 (At1g50680) plants showed a significant reduction in plant freezing tolerance compared to the wild type after chilling treatment. Homozygous ko3 (At5g13910) were less tolerant to high salinity than wild type plants. Our data suggest that At1g71450 is a negative regulator in ABA signaling, while At1g50680 and At5g13910 are positive regulators in cold and salt stress responses, respectively.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000247578500004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 3; ARTICLE; 13; 1746-1753; 52
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/250089]
专题	生命科学学院
推荐引用方式 GB/T 7714	Mei WenQian,Lei Juan,Xu Yu,et al. Characterization of three Arabidopsis AP2/EREBP family transcription factors involved in ABA sensitivity, freeze and salt tolerance[J]. 科学通报英文版,2007.
APA	Mei WenQian,Lei Juan,Xu Yu,Wei Gang,&Zhu YuXian.(2007).Characterization of three Arabidopsis AP2/EREBP family transcription factors involved in ABA sensitivity, freeze and salt tolerance.科学通报英文版.
MLA	Mei WenQian,et al."Characterization of three Arabidopsis AP2/EREBP family transcription factors involved in ABA sensitivity, freeze and salt tolerance".科学通报英文版 (2007).