Structural and functional comparison of MIF ortholog from Plasmodium   yoelii with MIF from its rodent host

doi:10.1016/j.molimm.2009.10.037

CORC > 北京大学 > 生命科学学院

	Structural and functional comparison of MIF ortholog from Plasmodium yoelii with MIF from its rodent host
	Shao, Dingding ; Zhong, Xiang ; Zhou, Yan-Feng ; Han, Zhifu ; Lin, Yahui ; Wang, Zhensheng ; Bu, Lingyi ; Zhang, Lianhui ; Su, Xiao-Dong ; Wang, Heng
刊名	分子免疫学
	2010
关键词	Macrophage migration inhibitory factor Malaria Tautomerase activity Crystal structure ERK1/2 MIGRATION-INHIBITORY FACTOR MALARIAL ANEMIA PARASITIC NEMATODE LEISHMANIA-MAJOR FACTOR HOMOLOG CELL-SURVIVAL FALCIPARUM PATHOGENESIS MACROPHAGES ACTIVATION
DOI	10.1016/j.molimm.2009.10.037
英文摘要	Host-derived macrophage migration inhibitory factor (MIF) has been implicated in the pathogenesis of malaria infection, especially in malarial anemia. Although two Plasmodium parasite-derived MIF orthologs, Plasmodium falciparum MIF and P. berghei MIF were identified recently, the crystal structure and the precise roles of Plasmodium-derived MIFs, particularly in combination with the host MIF, remain unknown. In this study, we identified another MIF ortholog from a rodent-specific P. yoelii (PyMIF). This molecule shares a conserved three-dimensional structure with murine MIF(MmMIF), but with a different substrate binding pattern and much lower tautomerase activity. It could activate host cells via several signaling pathways in vitro, and inhibiting macrophage apoptosis, also similarly to MmMIF. However, we found that PyMIF and MmMIF acted synergistically to activate the MAPK-ERK1/2 signaling pathway at very low concentration but acted antagonistically at higher concentration. Furthermore, we detected PyMIF in the sera of infected mice and found that injection of recombinant PyMIF (rPyMIF) during infection could up-regulate several pro-inflammatory cytokines in vivo and slightly delay the death of infected mice. These data suggest that PyMIF modulates host immune responses together with host MIF and has potential to prolong parasitemia or the chronicity of malaria infection. (C) 2010 Elsevier Ltd. All rights reserved.; Biochemistry & Molecular Biology; Immunology; SCI(E); 8; ARTICLE; 4; 726-737; 47
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/244872]
专题	生命科学学院
推荐引用方式 GB/T 7714	Shao, Dingding,Zhong, Xiang,Zhou, Yan-Feng,et al. Structural and functional comparison of MIF ortholog from Plasmodium yoelii with MIF from its rodent host[J]. 分子免疫学,2010.
APA	Shao, Dingding.,Zhong, Xiang.,Zhou, Yan-Feng.,Han, Zhifu.,Lin, Yahui.,...&Wang, Heng.(2010).Structural and functional comparison of MIF ortholog from Plasmodium yoelii with MIF from its rodent host.分子免疫学.
MLA	Shao, Dingding,et al."Structural and functional comparison of MIF ortholog from Plasmodium yoelii with MIF from its rodent host".分子免疫学 (2010).