AtFH8 Is Involved in Root Development under Effect of Low-Dose   Latrunculin B in Dividing Cells

doi:10.1093/mp/ssq085

CORC > 北京大学 > 生命科学学院

	AtFH8 Is Involved in Root Development under Effect of Low-Dose Latrunculin B in Dividing Cells
	Xue, Xiu-Hua ; Guo, Chun-Qing ; Du, Fei ; Lu, Quan-Long ; Zhang, Chuan-Mao ; Ren, Hai-Yun
刊名	分子植物
	2011
关键词	Actin binding protein formin cell division root meristem lateral root initiation actin nucleator and bundler ACTIN-BINDING PROTEINS ARABIDOPSIS-THALIANA AUXIN TRANSPORT BUNDLING ACTIVITY FORMIN POLLEN GROWTH CYTOSKELETON MEMBRANE DOMAIN
DOI	10.1093/mp/ssq085
英文摘要	Formins have been paid much attention for their potent nucleating activity. However, the connection between the in vivo functions of AtFHs (Arabidopsis thaliana formin homologs) and their effects on actin organization is poorly understood. In this study, we characterized the bundling activity of AtFH8 in vitro and in vivo. Biochemical analysis showed that AtFH8(FH1FH2) could form dimers and bundle preformed actin filaments or induce stellar structures during actin polymerization. Expression of truncated forms of AtFH8 and immunolocalization analysis showed that AtFH8 localized primarily to nuclear envelope in interphase and to the new cell wall after cytokinesis, depending primarily on its N-terminal transmembrane domain. GUS histochemical staining showed AtFH8 was predominantly expressed in Arabidopsis root meristem, vasculature, and outgrowth points of lateral roots. The primary root growth and lateral root initiation of atfh8 could be decreased by latrunculin B (LatB). Analysis of the number of dividing cells in Arabidopsis root tips showed that much fewer dividing cells in Lat B-treated atfh8 plants than wild-type plants, which indicates that AtFH8 was involved in cell division. Actin cytoskeleton in root meristem of atfh8-1 was more sensitive to LatB treatment than that of wild-type. Altogether, our results indicate that AtFH8 is an actin filament nucleator and bundler that functions in cell division and root development.; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 16; ARTICLE; 2; 264-278; 4
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/240653]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xue, Xiu-Hua,Guo, Chun-Qing,Du, Fei,et al. AtFH8 Is Involved in Root Development under Effect of Low-Dose Latrunculin B in Dividing Cells[J]. 分子植物,2011.
APA	Xue, Xiu-Hua,Guo, Chun-Qing,Du, Fei,Lu, Quan-Long,Zhang, Chuan-Mao,&Ren, Hai-Yun.(2011).AtFH8 Is Involved in Root Development under Effect of Low-Dose Latrunculin B in Dividing Cells.分子植物.
MLA	Xue, Xiu-Hua,et al."AtFH8 Is Involved in Root Development under Effect of Low-Dose Latrunculin B in Dividing Cells".分子植物 (2011).